Regulation of steroidogenesis in subclones of the MA-10 mouse Leydig tumor cell line

M. W. Kilgore, F. F.G. Rommerts, K. W.A. Wirtz, D. M. Stocco

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


We have previously reported the isolation of a subclone of the MA-10 mouse Leydig tumor cell line (MA-10 LP) which secretes less than 10% of the steroid synthesized by the parent, accumulates comparable amounts of cAMP and has equivalent cholesterol side-chain cleavage activity as the parent population (Kilgore and Stocco (1989) Endocrinology 124, 1210-1216). In the present study we show that addition of exogenous sterol carrier protein 2 (SCP2) to isolated mitochondria was not able to overcome the deficient steroid response of MA-10 LP. We have also demonstrated that human chorionic gonadotropin (hCG)-stimulated cellular events which activate steroid production by subsequently isolated mitochondria require ongoing protein synthesis, release of intracellular calcium and are mediated through the calcium-calmodulin complex. Additionally, mitochondrial sonicates from hCG-stimulated parent cells were able to stimulate steroid production by intact mitochondria isolated from unstimulated parent cells, whereas sonicates from similarly treated MA-10 LP had no effect on steroid production in these mitochondria. Together these data suggest that hCG induces changes in the mitochondria of the parent stock which are not induced to the same extent in the mitochondria of MA-10 LP.

Original languageEnglish
Pages (from-to)9-16
Number of pages8
JournalMolecular and Cellular Endocrinology
Issue number1
StatePublished - Feb 12 1990

Bibliographical note

Funding Information:
We acknowledge the technical assistance of Ms. Debbie DeHaven Alberts. This work was supported by NIH grants HD-17481, HD-07271 and HD-00685. M.W.K. is the recipient of a NIH predoctoral fellowship.


  • Calcium
  • Leydig
  • MA-10
  • Mitochondria
  • Steroidogenesis
  • Sterol carrier protein 2

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology


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