65 Scopus citations

Abstract

The relationship between insulin-mediated glucose disposal and fasting insulin and triglyceride (TG) concentrations, plasma post-heparin lipoprotein lipase (PH-LPL) activity and mass, and adipose tissue LPL activity, mass, and mRNA content was defined in 19 non-diabetic men. Insulin-mediated glucose uptake [as assessed by determining the steady- state plasma glucose (SSPG) concentration during a continuous infusion of somatostatin, insulin, and glucose] was significantly correlated with fasting TG concentration (r = 0.54, p < 0.02), plasma PH-LPL activity (r = - 0.52, p < 0.03) and mass (r = -0.49, p< 0.03), and adipose tissue LPL mRNA content (r = -0.68, p < 0.001). Comparable relationships were also seen when fasting insulin concentration was substituted for SSPG. Although adipose tissue LPL and mass correlated with each other (r = 0.76, p < 0.001) in a fasting state, they were not related to any other variable measured. Using in vivo and molecular biology techniques, these data demonstrate that the more insulin resistant an individual, the lower the level of plasma PH-LPL activity and mass, and the higher the plasma TG concentration. Since lower concentrations of adipose tissue mRNA were also directly correlated with plasma PH-LPL mass (r = 0.57, p < 0.01), and inversely with plasma TG concentration (r = -0.68, p < 0.001) as well as SSPG (r = -0.68, p < 0.001), it can be postulated that the relationship between insulin resistance and LPL activity and plasma TG concentration is associated with the inability of insulin to stimulate the transcription or to increase the intracellular mRNA stability of adipose tissue LPL in insulin resistant individuals.

Original languageEnglish
Pages (from-to)850-858
Number of pages9
JournalDiabetologia
Volume40
Issue number7
DOIs
StatePublished - 1997

Bibliographical note

Funding Information:
stitute of Health (HL-08506, RR-00 070 and DK 39 176) and by the Office of Research and Development, Medical Research Service, Department of Veterans Affairs. Dr. Maheux was a Scholar of the Medical Research Council of Canada. Dr. Salman Azhar is a Research Scientist at the VA Palo Alto Health Care System. We thank Dr. John Ong for his technical assistance in assessing the LPL mass. The support of the GCRC staff and our editorial assistants are also acknowledged.

Keywords

  • Adipose tissue
  • Insulin resistance
  • Lipoprotein lipase
  • RNA messenger
  • Triglycerides

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

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