Release of Monoamines from Striatum of Rat and Mouse Evoked by Local Application of Potassium: Evaluation of a New In Vivo Electrochemical Technique

Greg A. Gerhardt, Greg M. Rose, Barry J. Hoffer

Research output: Contribution to journalArticlepeer-review

77 Scopus citations

Abstract

Abstract: Local application of K+ via micropressure‐ejection, coupled with in vivo electrochemical detection, was used to study stimulated release from monoaminergic nerve terminals in the striatum of anesthetized rats and mice. K ‐evoked releases were reversible, reproducible, and dose‐dependent. In contrast, releases of electroactive species could not be evoked by local ejection of Na+. The magnitudes and time courses of K+‐evoked releases recorded from the caudate nucleus of mice were greater than those seen in rats. Local application of nomifensine, a putative catecholamine reuptake blocker, augmented the magnitudes and time courses of K+‐evoked releases. Releases were also recorded from brain regions adjacentthe striatum; these signals were always smaller than those seen in the caudate nucleus and had amplitudes that showed good correspondence to the relative degree of dopaminergic input to these areas. These data, taken together with other information in the literature, suggest that this new technique is well suited for in situ studies of monoamine release and reuptake in intact animals.

Original languageEnglish
Pages (from-to)842-850
Number of pages9
JournalJournal of Neurochemistry
Volume46
Issue number3
DOIs
StatePublished - Mar 1986

Funding

FundersFunder number
National Institute of Environmental Health Sciences (NIEHS)R01ES002011

    Keywords

    • 3,4‐Dihydroxyphenylethylamine
    • Caudate nucleus
    • In vivo electrochemistry
    • Mice
    • Potassium
    • Rats

    ASJC Scopus subject areas

    • Biochemistry
    • Cellular and Molecular Neuroscience

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