TY - JOUR
T1 - Release of Monoamines from Striatum of Rat and Mouse Evoked by Local Application of Potassium
T2 - Evaluation of a New In Vivo Electrochemical Technique
AU - Gerhardt, Greg A.
AU - Rose, Greg M.
AU - Hoffer, Barry J.
PY - 1986/3
Y1 - 1986/3
N2 - Abstract: Local application of K+ via micropressure‐ejection, coupled with in vivo electrochemical detection, was used to study stimulated release from monoaminergic nerve terminals in the striatum of anesthetized rats and mice. K − ‐evoked releases were reversible, reproducible, and dose‐dependent. In contrast, releases of electroactive species could not be evoked by local ejection of Na+. The magnitudes and time courses of K+‐evoked releases recorded from the caudate nucleus of mice were greater than those seen in rats. Local application of nomifensine, a putative catecholamine reuptake blocker, augmented the magnitudes and time courses of K+‐evoked releases. Releases were also recorded from brain regions adjacentthe striatum; these signals were always smaller than those seen in the caudate nucleus and had amplitudes that showed good correspondence to the relative degree of dopaminergic input to these areas. These data, taken together with other information in the literature, suggest that this new technique is well suited for in situ studies of monoamine release and reuptake in intact animals.
AB - Abstract: Local application of K+ via micropressure‐ejection, coupled with in vivo electrochemical detection, was used to study stimulated release from monoaminergic nerve terminals in the striatum of anesthetized rats and mice. K − ‐evoked releases were reversible, reproducible, and dose‐dependent. In contrast, releases of electroactive species could not be evoked by local ejection of Na+. The magnitudes and time courses of K+‐evoked releases recorded from the caudate nucleus of mice were greater than those seen in rats. Local application of nomifensine, a putative catecholamine reuptake blocker, augmented the magnitudes and time courses of K+‐evoked releases. Releases were also recorded from brain regions adjacentthe striatum; these signals were always smaller than those seen in the caudate nucleus and had amplitudes that showed good correspondence to the relative degree of dopaminergic input to these areas. These data, taken together with other information in the literature, suggest that this new technique is well suited for in situ studies of monoamine release and reuptake in intact animals.
KW - 3,4‐Dihydroxyphenylethylamine
KW - Caudate nucleus
KW - In vivo electrochemistry
KW - Mice
KW - Potassium
KW - Rats
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U2 - 10.1111/j.1471-4159.1986.tb13048.x
DO - 10.1111/j.1471-4159.1986.tb13048.x
M3 - Article
C2 - 3950610
AN - SCOPUS:0022626242
SN - 0022-3042
VL - 46
SP - 842
EP - 850
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
IS - 3
ER -