Relocation of the t-SNARE SNAP-23 from lamellipodia-like cell surface projections regulates compound exocytosis in mast cells

Zhenheng Guo; Christopher Turner; David Castle

doi:10.1016/S0092-8674(00)81594-9

Relocation of the t-SNARE SNAP-23 from lamellipodia-like cell surface projections regulates compound exocytosis in mast cells

Zhenheng Guo
, Christopher Turner
, David Castle

Pharmacology and Nutritional Sciences

Research output: Contribution to journal › Article › peer-review

237 Scopus citations

Abstract

For regulated secretion, mast cells and several other cell types utilize compound exocytosis, a combination of granule-plasma membrane and granule- granule fusions. The molecular machinery that controls this massive export process has not been identified. We report that SNAP-23, a t-SNARE related to SNAP-25, relocates in response to stimulation from plasma membrane lamellipodia-like projections to granule membranes in permeabilized mast cells. While relocation is a prerequisite for secretion, it can occur without membrane fusion and will expedite a subsequent secretory response. After relocation, SNAP-23 is required for exocytosis, implying a crucial role in promoting membrane fusion. Thus, relocation of this SNARE regulates compound exocytosis and links granuleplasma membrane and granule-granule fusions.

Original language	English
Pages (from-to)	537-548
Number of pages	12
Journal	Cell
Volume	94
Issue number	4
DOIs	https://doi.org/10.1016/S0092-8674(00)81594-9
State	Published - Aug 21 1998

Bibliographical note

Funding Information:
We thank Drs. Pietro DeCamilli, Judith White, and Samuel Green for reading this manuscript; Drs. Carl Creutz, Samuel Green, Anna Castle, and members of the Castle lab for valuable discussion. We are grateful to Drs. Pam Tuma, Ann Hubbard, Pietro DeCamilli, Reinhard Jahn, and Michael Caplan for providing antibodies; to Jan Redick and Bonnie Sheppard (Electron Microscope Facility) for providing cell sections for immunogold labeling; and to the UVA Biomolecular Research Facility for peptide synthesis. Special thanks to Dr. Theo Wu for initial Western blotting to identify SNAREs in mast cells. These studies were supported by a grant from the National Institutes of Health (DE09655).

Funding

We thank Drs. Pietro DeCamilli, Judith White, and Samuel Green for reading this manuscript; Drs. Carl Creutz, Samuel Green, Anna Castle, and members of the Castle lab for valuable discussion. We are grateful to Drs. Pam Tuma, Ann Hubbard, Pietro DeCamilli, Reinhard Jahn, and Michael Caplan for providing antibodies; to Jan Redick and Bonnie Sheppard (Electron Microscope Facility) for providing cell sections for immunogold labeling; and to the UVA Biomolecular Research Facility for peptide synthesis. Special thanks to Dr. Theo Wu for initial Western blotting to identify SNAREs in mast cells. These studies were supported by a grant from the National Institutes of Health (DE09655).

Funders	Funder number
National Institutes of Health (NIH)
National Institute of Dental and Craniofacial Research	R01DE009655
National Institute of Dental and Craniofacial Research

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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title = "Relocation of the t-SNARE SNAP-23 from lamellipodia-like cell surface projections regulates compound exocytosis in mast cells",

abstract = "For regulated secretion, mast cells and several other cell types utilize compound exocytosis, a combination of granule-plasma membrane and granule- granule fusions. The molecular machinery that controls this massive export process has not been identified. We report that SNAP-23, a t-SNARE related to SNAP-25, relocates in response to stimulation from plasma membrane lamellipodia-like projections to granule membranes in permeabilized mast cells. While relocation is a prerequisite for secretion, it can occur without membrane fusion and will expedite a subsequent secretory response. After relocation, SNAP-23 is required for exocytosis, implying a crucial role in promoting membrane fusion. Thus, relocation of this SNARE regulates compound exocytosis and links granuleplasma membrane and granule-granule fusions.",

author = "Zhenheng Guo and Christopher Turner and David Castle",

note = "Funding Information: We thank Drs. Pietro DeCamilli, Judith White, and Samuel Green for reading this manuscript; Drs. Carl Creutz, Samuel Green, Anna Castle, and members of the Castle lab for valuable discussion. We are grateful to Drs. Pam Tuma, Ann Hubbard, Pietro DeCamilli, Reinhard Jahn, and Michael Caplan for providing antibodies; to Jan Redick and Bonnie Sheppard (Electron Microscope Facility) for providing cell sections for immunogold labeling; and to the UVA Biomolecular Research Facility for peptide synthesis. Special thanks to Dr. Theo Wu for initial Western blotting to identify SNAREs in mast cells. These studies were supported by a grant from the National Institutes of Health (DE09655).",

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N2 - For regulated secretion, mast cells and several other cell types utilize compound exocytosis, a combination of granule-plasma membrane and granule- granule fusions. The molecular machinery that controls this massive export process has not been identified. We report that SNAP-23, a t-SNARE related to SNAP-25, relocates in response to stimulation from plasma membrane lamellipodia-like projections to granule membranes in permeabilized mast cells. While relocation is a prerequisite for secretion, it can occur without membrane fusion and will expedite a subsequent secretory response. After relocation, SNAP-23 is required for exocytosis, implying a crucial role in promoting membrane fusion. Thus, relocation of this SNARE regulates compound exocytosis and links granuleplasma membrane and granule-granule fusions.

AB - For regulated secretion, mast cells and several other cell types utilize compound exocytosis, a combination of granule-plasma membrane and granule- granule fusions. The molecular machinery that controls this massive export process has not been identified. We report that SNAP-23, a t-SNARE related to SNAP-25, relocates in response to stimulation from plasma membrane lamellipodia-like projections to granule membranes in permeabilized mast cells. While relocation is a prerequisite for secretion, it can occur without membrane fusion and will expedite a subsequent secretory response. After relocation, SNAP-23 is required for exocytosis, implying a crucial role in promoting membrane fusion. Thus, relocation of this SNARE regulates compound exocytosis and links granuleplasma membrane and granule-granule fusions.

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Relocation of the t-SNARE SNAP-23 from lamellipodia-like cell surface projections regulates compound exocytosis in mast cells

Abstract

Bibliographical note

Funding

ASJC Scopus subject areas

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