Dithiothreitol-dependent MgATP2 and MgADP- hydrolytic activities owing to nucleoside triphosphate hydrolase were examined kinetically un tachyzoite cells of five strains of Toxoplasma gondii with various virulence. The tachyzoites of all the strains were revealed to have a high ATP and ADP hydrolytic potency. The value of Vmax and Km obtained from each strain indicated that there were two classified types of T. gondii about ATP and ADP hydrolysis. The most virulent strain (RH) and avirulent strain (Fukaya) were classified in a type with higher ATPase than ADPase activities. Two virulent strains (C56, Beverley) and an avirulent strain (ME49) were classified in the other type with the same activities of ATPase and ADPase.
|Number of pages||4|
|Journal||FEMS Microbiology Letters|
|State||Published - Oct 1990|
Bibliographical noteFunding Information:
Tb~s wor~, wa~ supported in part by Grant-in-Aid for Encouragement of Young Scientists 01770266 from the Ministry of Education, Science a,'~d Culture of Japan, and also supported in part by a grant from Ohm,a rea Health Foundation. We thank Dr. M. Kobayashi (Chiba University) 1or providing us a computer program for K,, and V,,,~ determination.
- Nucleoside triphosphate hydrolase
- Toxoplasma gondii
ASJC Scopus subject areas
- Molecular Biology