Remodeling of HDL remnants generated by scavenger receptor class B type I

Nancy R. Webb, Maria C. De Beer, Bela F. Asztalos, Nathan Whitaker, Deneys R. Van Der Westhuyzen, Frederick C. De Beer

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


Scavenger receptor class B type I (SR-BI) mediates the selective transfer of cholesteryl ester from HDL to cells. We previously established that SR-BI overexpressed in livers of apolipoprotein A-I-deficient mice processes exogenous human HDL2 to incrementally smaller HDL particles. When mixed with normal mouse plasma either in vivo or ex vivo, SR-BI-generated HDL "remnants" rapidly remodel to form HDL-sized lipoproteins. In this study, we analyzed HDLs throughout the process of HDL remnant formation and investigated the mechanism of conversion to larger particles. Upon interacting with SR-BI, α-migrating HDL2 is initially converted to a preα-migrating particle that is ultimately processed to a smaller α-migrating HDL remnant. SR-BI does not appear to generate preβ-1 HDL particles. When incubated with isolated lipoprotein fractions, HDL remnants are converted to lipoprotein particles corresponding in size to the particle incubated with the HDL remnant. HDL remnant conversion is not altered in phospholipid transfer protein (PLTP)-deficient mouse plasma or by the addition of purified PLTP. Although LCAT-deficient plasma promoted only partial conversion, this deficiency was attributable to the nature of HDL particles in LCAT-/- mice rather than to a requirement for LCAT in the remodeling process. We conclude that HDL remnants, generated by SR-BI, are converted to larger particles by rapidly reassociating with existing HDL particles in an enzyme-independent manner.

Original languageEnglish
Pages (from-to)1666-1673
Number of pages8
JournalJournal of Lipid Research
Issue number9
StatePublished - Sep 2004

Bibliographical note

Copyright 2008 Elsevier B.V., All rights reserved.


  • Cholesteryl ester
  • High density lipoprotein
  • Selective lipid uptake

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology


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