Removal of psoralen monoadducts and crosslinks by human cell free extracts

Joyce T. Reardon; Peter Spielmann; Juch chin Huang; Srinivas Sastry; Aziz Sancar; John E. Hearst

doi:10.1093/nar/19.17.4623

Removal of psoralen monoadducts and crosslinks by human cell free extracts

Joyce T. Reardon, Peter Spielmann, Juch chin Huang, Srinivas Sastry, Aziz Sancar, John E. Hearst

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

Human cell free extracts are capable of carrying out damage-induced DNA synthesis in response to DNA damage by UV, psoralen, and cisplatin. We show that this damage-induced DNA synthesis is associated with removal of psoralen adducts and therefore is 'repair synthesis' and not an aberrant DNA synthesis reaction potentiated by DNA deformed by adducts. By comparing the denaturable fraction of psoralen adducted DNA which becomes labeled in the repair reaction to that of terminally labeled DNA (without repair) we have found that all DNA synthesis induced by psoralen monoadducts is the consequence of removal of these adducts. By the same approach we have obtained preliminary evidence that this in vitro system is capable of removing psoralen crosslinks as well.

Original language	English
Pages (from-to)	4623-4629
Number of pages	7
Journal	Nucleic Acids Research
Volume	19
Issue number	17
DOIs	https://doi.org/10.1093/nar/19.17.4623
State	Published - Sep 11 1991

Bibliographical note

Funding Information:
We thank J.J.Lin for critical reading of this manuscript. This work has been supported by the NIH grant GM32833, the Department of Energy grant DE-AcO3-76SF-OOO98 and in part by the grant CTR1872A from the Council for Tobacco Research-USA, Inc.; JTR was supported by fellowship ES05513-02 from the NIH.

ASJC Scopus subject areas

Genetics

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10.1093/nar/19.17.4623

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title = "Removal of psoralen monoadducts and crosslinks by human cell free extracts",

abstract = "Human cell free extracts are capable of carrying out damage-induced DNA synthesis in response to DNA damage by UV, psoralen, and cisplatin. We show that this damage-induced DNA synthesis is associated with removal of psoralen adducts and therefore is 'repair synthesis' and not an aberrant DNA synthesis reaction potentiated by DNA deformed by adducts. By comparing the denaturable fraction of psoralen adducted DNA which becomes labeled in the repair reaction to that of terminally labeled DNA (without repair) we have found that all DNA synthesis induced by psoralen monoadducts is the consequence of removal of these adducts. By the same approach we have obtained preliminary evidence that this in vitro system is capable of removing psoralen crosslinks as well.",

author = "Reardon, {Joyce T.} and Peter Spielmann and Huang, {Juch chin} and Srinivas Sastry and Aziz Sancar and Hearst, {John E.}",

note = "Funding Information: We thank J.J.Lin for critical reading of this manuscript. This work has been supported by the NIH grant GM32833, the Department of Energy grant DE-AcO3-76SF-OOO98 and in part by the grant CTR1872A from the Council for Tobacco Research-USA, Inc.; JTR was supported by fellowship ES05513-02 from the NIH.",

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AU - Reardon, Joyce T.

AU - Spielmann, Peter

AU - Huang, Juch chin

AU - Sastry, Srinivas

AU - Sancar, Aziz

AU - Hearst, John E.

N1 - Funding Information: We thank J.J.Lin for critical reading of this manuscript. This work has been supported by the NIH grant GM32833, the Department of Energy grant DE-AcO3-76SF-OOO98 and in part by the grant CTR1872A from the Council for Tobacco Research-USA, Inc.; JTR was supported by fellowship ES05513-02 from the NIH.

PY - 1991/9/11

Y1 - 1991/9/11

N2 - Human cell free extracts are capable of carrying out damage-induced DNA synthesis in response to DNA damage by UV, psoralen, and cisplatin. We show that this damage-induced DNA synthesis is associated with removal of psoralen adducts and therefore is 'repair synthesis' and not an aberrant DNA synthesis reaction potentiated by DNA deformed by adducts. By comparing the denaturable fraction of psoralen adducted DNA which becomes labeled in the repair reaction to that of terminally labeled DNA (without repair) we have found that all DNA synthesis induced by psoralen monoadducts is the consequence of removal of these adducts. By the same approach we have obtained preliminary evidence that this in vitro system is capable of removing psoralen crosslinks as well.

AB - Human cell free extracts are capable of carrying out damage-induced DNA synthesis in response to DNA damage by UV, psoralen, and cisplatin. We show that this damage-induced DNA synthesis is associated with removal of psoralen adducts and therefore is 'repair synthesis' and not an aberrant DNA synthesis reaction potentiated by DNA deformed by adducts. By comparing the denaturable fraction of psoralen adducted DNA which becomes labeled in the repair reaction to that of terminally labeled DNA (without repair) we have found that all DNA synthesis induced by psoralen monoadducts is the consequence of removal of these adducts. By the same approach we have obtained preliminary evidence that this in vitro system is capable of removing psoralen crosslinks as well.

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Removal of psoralen monoadducts and crosslinks by human cell free extracts

Abstract

Bibliographical note

ASJC Scopus subject areas

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