Renaturation of complementary DNA strands by herpes simplex virus type 1 ICP8

R. E. Dutch, I. R. Lehman

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

ICP8, the major single-stranded DNA-binding protein of herpes simplex virus type 1, promotes renaturation of complementary single strands of DNA. This reaction is ATP independent but requires Mg2+. The activity is maximal at pH 7.6 and 80 mM NaCl. The major product of the reaction is double-stranded DNA, and no evidence of large DNA networks is seen. The reaction occurs at subsaturating concentrations of ICP8 but reaches maximal levels with saturating concentrations of ICP8. Finally, the renaturation reaction is second order with respect to DNA concentration. The ability of ICP8 to promote the renaturation of complementary single strands suggests a role for ICP8 in the high level of recombination seen in cells infected with herpes simplex virus type 1.

Original languageEnglish
Pages (from-to)6945-6949
Number of pages5
JournalJournal of Virology
Volume67
Issue number12
StatePublished - Dec 1993

Funding

FundersFunder number
National Institute of Allergy and Infectious DiseasesR01AI026538

    ASJC Scopus subject areas

    • Microbiology
    • Immunology
    • Insect Science
    • Virology

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