Repetitive somatic embryogenesis from peanut cultures in liquid medium

Richard E. Durham, Wayne A. Parrott

Research output: Contribution to journalArticlepeer-review

61 Scopus citations

Abstract

A regeneration system based on repetitive somatic embryogenesis was developed for peanut (Arachis hypogaea L.). Embryogenic suspension cultures were initiated using individual somatic embryos induced from immature cotyledons cultured on a modified Murashige and Skoog medium containing 40 mg/l 2,4-D for 30 days. After transfer to a modified MS liquid medium, the somatic embryos produced masses of secondary and tertiary embryos which continued to proliferate following manual separation and subculture of the embryogenic clumps. The cultures exhibited exponential growth, and have been maintained for over one year without apparent loss of embryogenic potential. Further embryo development, germination, and conversion were achieved by placing embryo clumps onto hormone-free, solid medium. The inclusion of a desiccation period during embryo development enhanced conversion four-fold. Plants have been established in soil and appear to be phenotypically normal.

Original languageEnglish
Pages (from-to)122-125
Number of pages4
JournalPlant Cell Reports
Volume11
Issue number3
DOIs
StatePublished - Apr 1992

Keywords

  • Arachis hypogaea
  • Regeneration
  • Tissue culture

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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