Resident muscle stem cells are not required for testosterone-induced skeletal muscle hypertrophy

Davis A. Englund, Bailey D. Peck, Kevin A. Murach, Ally C. Neal, Hannah A. Caldwell, John J. McCarthy, Charlotte A. Peterson, Esther E. Dupont-Versteegden

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

It is postulated that testosterone-induced skeletal muscle hypertrophy is driven by myonuclear accretion as the result of satellite cell fusion. To directly test this hypothesis, we utilized the Pax7-DTA mouse model to deplete satellite cells in skeletal muscle followed by testosterone administration. Pax7-DTA mice (6 mo of age) were treated for 5 days with either vehicle [satellite cell replete (SC+)] or tamoxifen [satellite cell depleted (SC-)]. Following a washout period, a testosterone propionate or sham pellet was implanted for 21 days. Testosterone administration caused a significant increase in muscle fiber cross-sectional area in SC+ and SC- mice in both oxidative (soleus) and glycolytic (plantaris and extensor digitorum longus) muscles. In SC+ mice treated with testosterone, there was a significant increase in both satellite cell abundance and myonuclei that was completely absent in testosterone-treated SC- mice. These findings provide direct evidence that testosterone-induced muscle fiber hypertrophy does not require an increase in satellite cell abundance or myonuclear accretion. Listen to a podcast about this Rapid Report with senior author E. E. Dupont-Versteegden (https://ajpcell.podbean.com/e/podcast-on-paper-that-shows-testosterone-induced-skeletal-muscle-hypertrophy-does-not-need-muscle-stem-cells/).

Original languageEnglish
Pages (from-to)C719-C724
JournalAmerican Journal of Physiology - Cell Physiology
Volume317
Issue number4
DOIs
StatePublished - 2019

Bibliographical note

Publisher Copyright:
Copyright © 2019 the American Physiological Society

Keywords

  • Hypertrophy
  • Satellite cell
  • Skeletal muscle
  • Stem cell
  • Testosterone

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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