Resolution of Lipoprotein Subclasses by Charge Detection Mass Spectrometry

Corinne A. Lutomski, Scott M. Gordon, Alan T. Remaley, Martin F. Jarrold

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Lipoproteins are micelle-like assemblies that are key players in the pathogenesis of atherosclerosis. High-density lipoprotein (HDL), low-density lipoproteins (LDL), and very low density lipoprotein (VLDL) are the three major classes present in fasting plasma. Within each class, there is a broad size distribution with wide variations in protein and lipid content. The development of better metrics for cardiovascular risk is thought to depend on better characterization of lipoprotein subclasses. Using charge detection mass spectrometry (CDMS), the mass distributions of HDL, LDL, and VLDL have been directly measured for the first time. In the case of HDL, seven distinct subpopulations were resolved using a two-dimensional correlation of charge and mass. The resolved components are assigned to HDL particles containing different numbers of the key structural proteins apolipoprotein A-I and apolipoprotein A-II.

Original languageEnglish
Pages (from-to)6353-6356
Number of pages4
JournalAnalytical Chemistry
Volume90
Issue number11
DOIs
StatePublished - Jun 5 2018

Bibliographical note

Funding Information:
This material is based on work supported by the National Science Foundation under Grant CHE-1531823.

Publisher Copyright:
Copyright © 2018 American Chemical Society.

ASJC Scopus subject areas

  • Analytical Chemistry

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