Response of calceolaria × herbeohybrida cultivars to substrate ph and corresponding leaf tissue nutrient concentration effects

Calceolaria (Calceolaria×herbeohybrida) is a flowering potted greenhouse crop that often develops upper-leaf chlorosis, interveinal chlorosis, and marginal and leaf-tip necrosis (death) caused by cultural practices. The objectives of this research were to 1) determine the optimal incorporation rate of dolomitic and/or hydrated lime to increase substrate pH; 2) determine the influence of the liming material on substrate pH, plant growth, and leaf tissue nutrient concentrations; and 3) determine the optimal substrate pH to grow and maintain during calceolaria production. Sphagnum peatmoss was amended with 20% (by volume) perlite and incorporated with pulverized dolomitic carbonate limestone (DL) and/or hydrated limestone (HL) at the following concentrations: 48.1 kg m^-3 or 144.2 kg m^-3 DL, 17.6 kg m^-3 DL + 5.3 kg m^-3 HL, or 17.6 kg m^-3 DL + 10.6 kg m^-3 HL to achieve a target substrate pH of 4.5, 5.5, 6.5, and 7.5, respectively. Calceolaria 'Orange', 'Orange Red Eye', 'Yellow', and 'Yellow Red Eye' were grown in each of the prepared substrates. For all cultivars, substrate solution pH increased as limestone incorporation concentration and weeks after transplant (WAT) increased, although to different magnitudes. For example, as limestone incorporation increased from 48.1 kg m^-3 DL to 17.6 kg m^-3 DL + 10.6 kg m^-3 HL, substrate solution pH for 'Orange' calceolaria increased from 4.1 to 6.9 to 4.8 to 7.2 at 2 and 6 WAT, respectively. Substrate solution electrical conductivity (EC) and growth indices were not influenced by limestone incorporation, but total plant dry mass increased. Few macronutrients and most micronutrients were influenced by limestone incorporation. Leaf tissue iron concentrations for 'Orange', 'Orange Red Eye', 'Yellow', and 'Yellow Red Eye' calceolaria decreased by 146%, 91%, 71%, and 84%, respectively, when plants were grown in substrates incorporated with increasing limestone concentrations from 144.2 kg m^-3 DL to 17.6 kg m^-3 DL + 10.6 kg m^-3 HL (pH 6.5-6.9). Therefore, incorporating 144.2 kg m^-3 DL into peat-based substrates and maintaining a pH <6.5 will avoid high pH-induced Fe deficiency and prevent upper-leaf and interveinal chlorosis.