Purpose: To assess the cytotoxicity of 35% hydrogen peroxide (HP) bleaching gel applied for 15 min to sound or restored teeth with two-step self-etching adhesive systems and composite resin. Materials and Methods: Sound and restored enamel/dentin disks were stored in water for 24 h or 6 months + thermocycling. The disks were adapted to artificial pulp chambers and placed in compartments containing culture medium. Immediately after bleaching, the culture medium in contact with dentin was applied for 1 h to previously cultured odontoblast-like MDPC-23 cells. Thereafter, cell viability (MTT assay) and morphology (SEM) were assessed. Data were analyzed by two-way ANOVA and Tukey's test (α = 5%). Results: In comparison to the negative control group (no treatment), no significant cell viability reduction occurred in those groups in which sound teeth were bleached. However, a significant decrease in cell viability was observed in the adhesive-restored bleached groups compared to negative control. No significant difference among bleached groups was observed with respect to the presence of restoration and storage time. Conclusion: The application of 35% HP bleaching gel to sound teeth for 15 min does not cause toxic effects in pulp cells. When this bleaching protocol was performed in adhesive-restored teeth, a significant toxic effect occurred.
|Number of pages||7|
|Journal||Journal of Adhesive Dentistry|
|State||Published - 2015|
Bibliographical noteFunding Information:
This study was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo-FAPESP (grant # 2013/23520-0), Conselho Nacional de Desenvolvimento Científico e Tecnológico-CNPq (grant # 301291/2010-1), and FUNDUNESP (grant # 0024/021/13-PROPE-CDC).
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- Laboratory research
- Tooth bleaching
ASJC Scopus subject areas
- Oral Surgery