Responses of dental pulp cells to a less invasive bleaching technique applied to adhesively restored teeth

Diana Gabriela Soares, Nancy Tomoko Sacono, Ana Paula Dias Ribeiro, Fernanda Gonçalves Basso, Débora Sales Scheffel, Josimeri Hebling, Carlos Alberto de Souza Costa

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Purpose: To assess the cytotoxicity of 35% hydrogen peroxide (HP) bleaching gel applied for 15 min to sound or restored teeth with two-step self-etching adhesive systems and composite resin. Materials and Methods: Sound and restored enamel/dentin disks were stored in water for 24 h or 6 months + thermocycling. The disks were adapted to artificial pulp chambers and placed in compartments containing culture medium. Immediately after bleaching, the culture medium in contact with dentin was applied for 1 h to previously cultured odontoblast-like MDPC-23 cells. Thereafter, cell viability (MTT assay) and morphology (SEM) were assessed. Data were analyzed by two-way ANOVA and Tukey's test (α = 5%). Results: In comparison to the negative control group (no treatment), no significant cell viability reduction occurred in those groups in which sound teeth were bleached. However, a significant decrease in cell viability was observed in the adhesive-restored bleached groups compared to negative control. No significant difference among bleached groups was observed with respect to the presence of restoration and storage time. Conclusion: The application of 35% HP bleaching gel to sound teeth for 15 min does not cause toxic effects in pulp cells. When this bleaching protocol was performed in adhesive-restored teeth, a significant toxic effect occurred.

Original languageEnglish
Pages (from-to)155-161
Number of pages7
JournalJournal of Adhesive Dentistry
Issue number2
StatePublished - 2015

Bibliographical note

Publisher Copyright:
© Quintessenz. All right reserved.


  • Adhesives
  • Laboratory research
  • Odontoblasts
  • Tooth bleaching
  • Toxicity

ASJC Scopus subject areas

  • Orthodontics
  • Oral Surgery
  • Periodontics


Dive into the research topics of 'Responses of dental pulp cells to a less invasive bleaching technique applied to adhesively restored teeth'. Together they form a unique fingerprint.

Cite this