Reusing nylon membranes for radioactive hybridizations

D. W. Thornbury, M. L. Farman

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


We describe a procedure for recycling nylon hybridization membranes, enabling their repeated use for radioactive Southern hybridization analysis of different DNA samples. Following hybridization and probe removal, nylon membranes containing covalently linked DNAs were treated with 0.55% sodium hypochlorite. This destroyed the DNA, thereby preventing it from participating in further hybridization and enabling the membranes to be used subsequently for binding new DNA samples. With this procedure, we were able to reuse a single membrane as many as 13 times, with no detectable loss in signal. This method was shown to be effective for membranes supplied by three different manufacturers.

Original languageEnglish
Pages (from-to)1250-1254
Number of pages5
Issue number6
StatePublished - 2000

ASJC Scopus subject areas

  • Biotechnology
  • General Biochemistry, Genetics and Molecular Biology


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