Rho GTPase activity modulates paramyxovirus fusion protein-mediated cell-cell fusion

Rachel M. Schowalter; Mark A. Wurth; Hector C. Aguilar; Benhur Lee; Carole L. Moncman; Richard O. McCann; Rebecca Ellis Dutch

doi:10.1016/j.virol.2006.01.033

Rho GTPase activity modulates paramyxovirus fusion protein-mediated cell-cell fusion

Rachel M. Schowalter, Mark A. Wurth, Hector C. Aguilar, Benhur Lee, Carole L. Moncman, Richard O. McCann, Rebecca Ellis Dutch

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

The paramyxovirus fusion protein (F) promotes fusion of the viral envelope with the plasma membrane of target cells as well as cell-cell fusion. The plasma membrane is closely associated with the actin cytoskeleton, but the role of actin dynamics in paramyxovirus F-mediated membrane fusion is unclear. We examined cell-cell fusion promoted by two different paramyxovirus F proteins in three cell types in the presence of constitutively active Rho family GTPases, major cellular coordinators of actin dynamics. Reporter gene and syncytia assays demonstrated that expression of either Rac1^V12 or Cdc42^V12 could increase cell-cell fusion promoted by the Hendra or SV5 glycoproteins, though the effect was dependent on the cell type expressing the viral glycoproteins. In contrast, RhoA^L63 decreased cell-cell fusion promoted by Hendra glycoproteins but had little affect on SV5 F-mediated fusion. Also, data suggested that GTPase activation in the viral glycoprotein-containing cell was primarily responsible for changes in fusion. Additionally, we found that activated Cdc42 promoted nuclear rearrangement in syncytia.

Original language	English
Pages (from-to)	323-334
Number of pages	12
Journal	Virology
Volume	350
Issue number	2
DOIs	https://doi.org/10.1016/j.virol.2006.01.033
State	Published - Jul 5 2006

Bibliographical note

Funding Information:
We thank Lin-Fa Wang of the Australian Animal Health Laboratory for the Hendra virus F and G plasmids, Robert Lamb (HHMI, Northwestern University) for the pCAGGS-SV5 F and HN expression plasmids, Richard Randall (University of St Andrews, Fife, UK) for the SV5 F-specific antibodies, and Karl-Klaus Conzelmann (Max Pettenkofer Institut) for kindly providing the BSR cells. Also, we thank Doug Andres (University of Kentucky) for the pCMV5M-GTPase plasmids as well as for his helpful discussion. We are grateful to the members of the Dutch laboratory who critically reviewed the manuscript. This study was partially supported by NIH grants A51517 (R.E.D.) and COBRE-P20RR20171 (R.O.M.).

Funding

We thank Lin-Fa Wang of the Australian Animal Health Laboratory for the Hendra virus F and G plasmids, Robert Lamb (HHMI, Northwestern University) for the pCAGGS-SV5 F and HN expression plasmids, Richard Randall (University of St Andrews, Fife, UK) for the SV5 F-specific antibodies, and Karl-Klaus Conzelmann (Max Pettenkofer Institut) for kindly providing the BSR cells. Also, we thank Doug Andres (University of Kentucky) for the pCMV5M-GTPase plasmids as well as for his helpful discussion. We are grateful to the members of the Dutch laboratory who critically reviewed the manuscript. This study was partially supported by NIH grants A51517 (R.E.D.) and COBRE-P20RR20171 (R.O.M.).

Funders	Funder number
National Institutes of Health (NIH)	A51517, COBRE-P20RR20171
National Institute of Allergy and Infectious Diseases	R01AI051517

Keywords

Actin
Glycoprotein
Membrane fusion
Paramyxovirus
Rho GTPase

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2006.01.033

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@article{6347adfa220a4ea8a15bb3b6d3db7465,

title = "Rho GTPase activity modulates paramyxovirus fusion protein-mediated cell-cell fusion",

abstract = "The paramyxovirus fusion protein (F) promotes fusion of the viral envelope with the plasma membrane of target cells as well as cell-cell fusion. The plasma membrane is closely associated with the actin cytoskeleton, but the role of actin dynamics in paramyxovirus F-mediated membrane fusion is unclear. We examined cell-cell fusion promoted by two different paramyxovirus F proteins in three cell types in the presence of constitutively active Rho family GTPases, major cellular coordinators of actin dynamics. Reporter gene and syncytia assays demonstrated that expression of either Rac1V12 or Cdc42V12 could increase cell-cell fusion promoted by the Hendra or SV5 glycoproteins, though the effect was dependent on the cell type expressing the viral glycoproteins. In contrast, RhoAL63 decreased cell-cell fusion promoted by Hendra glycoproteins but had little affect on SV5 F-mediated fusion. Also, data suggested that GTPase activation in the viral glycoprotein-containing cell was primarily responsible for changes in fusion. Additionally, we found that activated Cdc42 promoted nuclear rearrangement in syncytia.",

keywords = "Actin, Glycoprotein, Membrane fusion, Paramyxovirus, Rho GTPase",

author = "Schowalter, {Rachel M.} and Wurth, {Mark A.} and Aguilar, {Hector C.} and Benhur Lee and Moncman, {Carole L.} and McCann, {Richard O.} and Dutch, {Rebecca Ellis}",

note = "Funding Information: We thank Lin-Fa Wang of the Australian Animal Health Laboratory for the Hendra virus F and G plasmids, Robert Lamb (HHMI, Northwestern University) for the pCAGGS-SV5 F and HN expression plasmids, Richard Randall (University of St Andrews, Fife, UK) for the SV5 F-specific antibodies, and Karl-Klaus Conzelmann (Max Pettenkofer Institut) for kindly providing the BSR cells. Also, we thank Doug Andres (University of Kentucky) for the pCMV5M-GTPase plasmids as well as for his helpful discussion. We are grateful to the members of the Dutch laboratory who critically reviewed the manuscript. This study was partially supported by NIH grants A51517 (R.E.D.) and COBRE-P20RR20171 (R.O.M.).",

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AU - Schowalter, Rachel M.

AU - Wurth, Mark A.

AU - Aguilar, Hector C.

AU - Lee, Benhur

AU - Moncman, Carole L.

AU - McCann, Richard O.

AU - Dutch, Rebecca Ellis

N1 - Funding Information: We thank Lin-Fa Wang of the Australian Animal Health Laboratory for the Hendra virus F and G plasmids, Robert Lamb (HHMI, Northwestern University) for the pCAGGS-SV5 F and HN expression plasmids, Richard Randall (University of St Andrews, Fife, UK) for the SV5 F-specific antibodies, and Karl-Klaus Conzelmann (Max Pettenkofer Institut) for kindly providing the BSR cells. Also, we thank Doug Andres (University of Kentucky) for the pCMV5M-GTPase plasmids as well as for his helpful discussion. We are grateful to the members of the Dutch laboratory who critically reviewed the manuscript. This study was partially supported by NIH grants A51517 (R.E.D.) and COBRE-P20RR20171 (R.O.M.).

PY - 2006/7/5

Y1 - 2006/7/5

N2 - The paramyxovirus fusion protein (F) promotes fusion of the viral envelope with the plasma membrane of target cells as well as cell-cell fusion. The plasma membrane is closely associated with the actin cytoskeleton, but the role of actin dynamics in paramyxovirus F-mediated membrane fusion is unclear. We examined cell-cell fusion promoted by two different paramyxovirus F proteins in three cell types in the presence of constitutively active Rho family GTPases, major cellular coordinators of actin dynamics. Reporter gene and syncytia assays demonstrated that expression of either Rac1V12 or Cdc42V12 could increase cell-cell fusion promoted by the Hendra or SV5 glycoproteins, though the effect was dependent on the cell type expressing the viral glycoproteins. In contrast, RhoAL63 decreased cell-cell fusion promoted by Hendra glycoproteins but had little affect on SV5 F-mediated fusion. Also, data suggested that GTPase activation in the viral glycoprotein-containing cell was primarily responsible for changes in fusion. Additionally, we found that activated Cdc42 promoted nuclear rearrangement in syncytia.

AB - The paramyxovirus fusion protein (F) promotes fusion of the viral envelope with the plasma membrane of target cells as well as cell-cell fusion. The plasma membrane is closely associated with the actin cytoskeleton, but the role of actin dynamics in paramyxovirus F-mediated membrane fusion is unclear. We examined cell-cell fusion promoted by two different paramyxovirus F proteins in three cell types in the presence of constitutively active Rho family GTPases, major cellular coordinators of actin dynamics. Reporter gene and syncytia assays demonstrated that expression of either Rac1V12 or Cdc42V12 could increase cell-cell fusion promoted by the Hendra or SV5 glycoproteins, though the effect was dependent on the cell type expressing the viral glycoproteins. In contrast, RhoAL63 decreased cell-cell fusion promoted by Hendra glycoproteins but had little affect on SV5 F-mediated fusion. Also, data suggested that GTPase activation in the viral glycoprotein-containing cell was primarily responsible for changes in fusion. Additionally, we found that activated Cdc42 promoted nuclear rearrangement in syncytia.

KW - Actin

KW - Glycoprotein

KW - Membrane fusion

KW - Paramyxovirus

KW - Rho GTPase

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Rho GTPase activity modulates paramyxovirus fusion protein-mediated cell-cell fusion

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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