The simultaneous elimination of organic waste and the production of clean fuels will have an immense impact on both the society and the industrial manufacturing sector. The enhanced understanding of the interface between nanoparticles and photo-responsive bacteria will further advance the knowledge of their interactions with biological systems. Although literature shows the production of gases by photobacteria, herein, we demonstrated the integration of photonics, biology, and nanostructured plasmonic materials for hydrogen production with a lower greenhouse CO2 gas content at quantified light energy intensity and wavelength. Phototrophic purple non-sulfur bacteria were able to generate hydrogen as a byproduct of nitrogen fixation using the energy absorbed from visible and near-IR (NIR) light. This type of biological hydrogen production has suffered from low efficiency of converting light energy into hydrogen in part due to light sources that do not exploit the organisms' capacity for NIR absorption. We used NIR light sources and optically resonant gold-silica core-shell nanoparticles to increase the light utilization of the bacteria to convert waste organic acids such as acetic and maleic acids to hydrogen. The batch growth studies for the small cultures (40 mL) of Rhodopseudomonas palustris demonstrated >2.5-fold increase in hydrogen production when grown under an NIR source (167 ± 18 μmol H2) compared to that for a broad-band light source (60 ± 6 μmol H2) at equal light intensity (130 W m-2). The addition of the mPEG-coated optically resonant gold-silica core-shell nanoparticles in the solution further improved the hydrogen production from 167 ± 18 to 398 ± 108 μmol H2 at 130 W m-2. The average hydrogen production rate with the nanoparticles was 127 ± 35 μmol L-1 h-1 at 130 W m-2.
|Number of pages||10|
|State||Published - 2019|
Bibliographical noteFunding Information:
This material was based upon work supported by the National Science Foundation EAGER grant 1700091 and by NSF EPSCoR grant 1355438. The NSF EAGER grant was awarded in February 2017. Additional nancial support was provided by Southern Company. This work was performed in part at the UK Center for Nanoscale Science and Engineering and the UK Electron Microscopy Center, which are members of the National Nanotechnology Coordinated Infrastructure (NNCI). NNCI is supported by the National Science Foundation (ECCS-1542164). This work used equipment supported by the National Science Foundation Grant No. CMMI-1125998.
© The Royal Society of Chemistry 2019.
ASJC Scopus subject areas
- Chemistry (all)
- Chemical Engineering (all)