TY - CHAP
T1 - Ribozyme-mediated trans insertion-splicing into target RNAs
AU - Dotson, P. Patrickii
AU - Hart, Jonathan
AU - Noe, Christopher
AU - Testa, Stephen M.
PY - 2012
Y1 - 2012
N2 - The trans insertion-splicing (TIS) reaction is a technique that can be used to site-specifically insert an RNA donor substrate into a separate RNA acceptor substrate. The TIS reaction, which is catalyzed by a group I intron-derived ribozyme from Pneumocystis carinii, is described with regards to system design, ribozyme preparation, and the overall protocol for conducting the TIS reaction.
AB - The trans insertion-splicing (TIS) reaction is a technique that can be used to site-specifically insert an RNA donor substrate into a separate RNA acceptor substrate. The TIS reaction, which is catalyzed by a group I intron-derived ribozyme from Pneumocystis carinii, is described with regards to system design, ribozyme preparation, and the overall protocol for conducting the TIS reaction.
KW - Group I intron
KW - Pneumocystis carinii
KW - Ribozyme
KW - Trans insertion-splicing
UR - http://www.scopus.com/inward/record.url?scp=84858216524&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84858216524&partnerID=8YFLogxK
U2 - 10.1007/978-1-61779-545-9_24
DO - 10.1007/978-1-61779-545-9_24
M3 - Chapter
C2 - 22315082
AN - SCOPUS:84858216524
SN - 9781617795442
T3 - Methods in Molecular Biology
SP - 385
EP - 394
BT - Ribozymes
A2 - Hartig, Jorg
ER -