Ribozyme-mediated trans insertion-splicing into target RNAs

P. Patrickii Dotson, Jonathan Hart, Christopher Noe, Stephen M. Testa

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations


The trans insertion-splicing (TIS) reaction is a technique that can be used to site-specifically insert an RNA donor substrate into a separate RNA acceptor substrate. The TIS reaction, which is catalyzed by a group I intron-derived ribozyme from Pneumocystis carinii, is described with regards to system design, ribozyme preparation, and the overall protocol for conducting the TIS reaction.

Original languageEnglish
Title of host publicationRibozymes
Subtitle of host publicationMethods and Protocols
EditorsJorg Hartig
Number of pages10
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Group I intron
  • Pneumocystis carinii
  • Ribozyme
  • Trans insertion-splicing

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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