Based on prior work, a cellulase from glycosyl hydrolase family 7 (GHF7) was identified and found to be expressed at a high level in Coptotermes formosanus. To determine the function of GHF7 family members in vivo, we used RNA interference (RNAi) to functionally analyse the exoglucanase gene Pseudotrichonympha grassii cellobiohydrolase gene (PgCBH), which was highly expressed in Pseudotrichonympha grassii, a flagellate found in the hindgut of C. formosanus. In this study, the expression level of PgCBH was down-regulated by RNAi, causing the death of P. grassii, but no effect was observed for other flagellates found in C. formosanus. RNAi also resulted in significantly reduced exoglucanase activity, and no effect was observed for endoglucanase and β-glucosidase activities. This result demonstrated that the PgCBH gene plays a role in the protist lignocellulolytic process and is also important for host survival. PgCBH can be used as a target gene and has potential as a bioinsecticide for use against termites.
|Number of pages||10|
|Journal||Insect Molecular Biology|
|State||Published - Apr 1 2017|
Bibliographical noteFunding Information:
This work was supported by grants from the National Natural Science Foundation of China (31472046, 31172153, 31300426, 31272037 and 31030060).
© 2016 The Royal Entomological Society
- Coptotermes formosanus
- Pseudotrichonympha grassii cellobiohydrolase gene (PgCBH)
ASJC Scopus subject areas
- Molecular Biology
- Insect Science