RNA interference unveils the importance of Pseudotrichonympha grassii cellobiohydrolase, a protozoan exoglucanase, in termite cellulose degradation

X. J. Liu, L. Xie, N. Liu, S. Zhan, X. G. Zhou, Q. Wang

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Based on prior work, a cellulase from glycosyl hydrolase family 7 (GHF7) was identified and found to be expressed at a high level in Coptotermes formosanus. To determine the function of GHF7 family members in vivo, we used RNA interference (RNAi) to functionally analyse the exoglucanase gene Pseudotrichonympha grassii cellobiohydrolase gene (PgCBH), which was highly expressed in Pseudotrichonympha grassii, a flagellate found in the hindgut of C. formosanus. In this study, the expression level of PgCBH was down-regulated by RNAi, causing the death of P. grassii, but no effect was observed for other flagellates found in C. formosanus. RNAi also resulted in significantly reduced exoglucanase activity, and no effect was observed for endoglucanase and β-glucosidase activities. This result demonstrated that the PgCBH gene plays a role in the protist lignocellulolytic process and is also important for host survival. PgCBH can be used as a target gene and has potential as a bioinsecticide for use against termites.

Original languageEnglish
Pages (from-to)233-242
Number of pages10
JournalInsect Molecular Biology
Volume26
Issue number2
DOIs
StatePublished - Apr 1 2017

Bibliographical note

Publisher Copyright:
© 2016 The Royal Entomological Society

Keywords

  • Coptotermes formosanus
  • Pseudotrichonympha grassii cellobiohydrolase gene (PgCBH)
  • RNAi
  • exoglucanase
  • flagellate

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Insect Science

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