RNAi assays in the striped flea beetle (Phyllotreta striolata) suggest Psγ-COPI and PsArf1COPI as potential molecular targets for pest control

Mujuan Guo; Ran Gao; Satyabrata Nanda; Yingqiu Li; Changfei Guo; Xuguo Zhou; Youjun Zhang; Chunxiao Yang; Huipeng Pan

doi:10.1016/j.pestbp.2023.105428

RNAi assays in the striped flea beetle (Phyllotreta striolata) suggest Psγ-COPI and PsArf1COPI as potential molecular targets for pest control

Mujuan Guo, Ran Gao, Satyabrata Nanda, Yingqiu Li, Changfei Guo, Xuguo Zhou, Youjun Zhang, Chunxiao Yang, Huipeng Pan

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Phyllotreta striolata (Fabricius), commonly known as the striped flea beetle (SFB), is a notorious insect pest that attacks Brassicaceae plants worldwide, leading to tremendous economic losses. RNA interference (RNAi) has been proposed as a promising strategy for sustainable and eco-friendly pest control. In this study, a total of nine housekeeping genes including PsVATPA, PsHSP90, PsEF1A, PsRPL6, PsRPS24, PsActin, PsTUBA, PsRPS18, and PsRPL4 were evaluated under four different conditions (organization, population, sex, and RNAi). PsEF1A and PsVATPA were identified as the best reference genes for RNAi bioassay. Furthermore, a total of 24 target genes were selected to investigate their RNAi effects in SFB adults with double-stranded RNAs (dsRNAs), five of them showed significant mortality (28.00% to 70.00%), namely Psα-COPI, Psβ-COPI, PsRPS18, Psγ-COPI, and PsArf1COPI. We found that gene transcript levels of the two most lethal genes, Psγ-COPI and PsArf1COPI, were significantly decreased after treated with the target dsRNAs either by feeding or injection method. The findings from this study demonstrated that the introduction of dsRNAs via oral feedings or injection induces the RNAi-mediated silencing of target genes and can lead to insect mortality. Overall, the identified target genes can be explored in developing RNAi-based insecticides for SFB control.

Original language	English
Article number	105428
Journal	Pesticide Biochemistry and Physiology
Volume	193
DOIs	https://doi.org/10.1016/j.pestbp.2023.105428
State	Published - Jun 2023

Bibliographical note

Publisher Copyright:
© 2023

Funding

This research was supported by the National Key Research and Development Program of China ( 2022YFD1401200 ; 2017YFD0200900 ), and a start-up fund from the South China Agricultural University . The granting agencies have no role in the study design, data collection, analysis, decision to publish, or preparation of the manuscript.

Funders	Funder number
National Key Basic Research and Development Program of China	2022YFD1401200, 2017YFD0200900
National Key Basic Research and Development Program of China
South China Agricultural University

Keywords

Feeding RNAi
Injection RNAi
Pest control
Phyllotreta striolata
RNA interference
Reference gene

ASJC Scopus subject areas

Agronomy and Crop Science
Health, Toxicology and Mutagenesis

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10.1016/j.pestbp.2023.105428

Cite this

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title = "RNAi assays in the striped flea beetle (Phyllotreta striolata) suggest Psγ-COPI and PsArf1COPI as potential molecular targets for pest control",

abstract = "Phyllotreta striolata (Fabricius), commonly known as the striped flea beetle (SFB), is a notorious insect pest that attacks Brassicaceae plants worldwide, leading to tremendous economic losses. RNA interference (RNAi) has been proposed as a promising strategy for sustainable and eco-friendly pest control. In this study, a total of nine housekeeping genes including PsVATPA, PsHSP90, PsEF1A, PsRPL6, PsRPS24, PsActin, PsTUBA, PsRPS18, and PsRPL4 were evaluated under four different conditions (organization, population, sex, and RNAi). PsEF1A and PsVATPA were identified as the best reference genes for RNAi bioassay. Furthermore, a total of 24 target genes were selected to investigate their RNAi effects in SFB adults with double-stranded RNAs (dsRNAs), five of them showed significant mortality (28.00\% to 70.00\%), namely Psα-COPI, Psβ-COPI, PsRPS18, Psγ-COPI, and PsArf1COPI. We found that gene transcript levels of the two most lethal genes, Psγ-COPI and PsArf1COPI, were significantly decreased after treated with the target dsRNAs either by feeding or injection method. The findings from this study demonstrated that the introduction of dsRNAs via oral feedings or injection induces the RNAi-mediated silencing of target genes and can lead to insect mortality. Overall, the identified target genes can be explored in developing RNAi-based insecticides for SFB control.",

keywords = "Feeding RNAi, Injection RNAi, Pest control, Phyllotreta striolata, RNA interference, Reference gene",

author = "Mujuan Guo and Ran Gao and Satyabrata Nanda and Yingqiu Li and Changfei Guo and Xuguo Zhou and Youjun Zhang and Chunxiao Yang and Huipeng Pan",

note = "Publisher Copyright: {\textcopyright} 2023",

year = "2023",

month = jun,

doi = "10.1016/j.pestbp.2023.105428",

language = "English",

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T1 - RNAi assays in the striped flea beetle (Phyllotreta striolata) suggest Psγ-COPI and PsArf1COPI as potential molecular targets for pest control

AU - Guo, Mujuan

AU - Gao, Ran

AU - Nanda, Satyabrata

AU - Li, Yingqiu

AU - Guo, Changfei

AU - Zhou, Xuguo

AU - Zhang, Youjun

AU - Yang, Chunxiao

AU - Pan, Huipeng

PY - 2023/6

Y1 - 2023/6

N2 - Phyllotreta striolata (Fabricius), commonly known as the striped flea beetle (SFB), is a notorious insect pest that attacks Brassicaceae plants worldwide, leading to tremendous economic losses. RNA interference (RNAi) has been proposed as a promising strategy for sustainable and eco-friendly pest control. In this study, a total of nine housekeeping genes including PsVATPA, PsHSP90, PsEF1A, PsRPL6, PsRPS24, PsActin, PsTUBA, PsRPS18, and PsRPL4 were evaluated under four different conditions (organization, population, sex, and RNAi). PsEF1A and PsVATPA were identified as the best reference genes for RNAi bioassay. Furthermore, a total of 24 target genes were selected to investigate their RNAi effects in SFB adults with double-stranded RNAs (dsRNAs), five of them showed significant mortality (28.00% to 70.00%), namely Psα-COPI, Psβ-COPI, PsRPS18, Psγ-COPI, and PsArf1COPI. We found that gene transcript levels of the two most lethal genes, Psγ-COPI and PsArf1COPI, were significantly decreased after treated with the target dsRNAs either by feeding or injection method. The findings from this study demonstrated that the introduction of dsRNAs via oral feedings or injection induces the RNAi-mediated silencing of target genes and can lead to insect mortality. Overall, the identified target genes can be explored in developing RNAi-based insecticides for SFB control.

AB - Phyllotreta striolata (Fabricius), commonly known as the striped flea beetle (SFB), is a notorious insect pest that attacks Brassicaceae plants worldwide, leading to tremendous economic losses. RNA interference (RNAi) has been proposed as a promising strategy for sustainable and eco-friendly pest control. In this study, a total of nine housekeeping genes including PsVATPA, PsHSP90, PsEF1A, PsRPL6, PsRPS24, PsActin, PsTUBA, PsRPS18, and PsRPL4 were evaluated under four different conditions (organization, population, sex, and RNAi). PsEF1A and PsVATPA were identified as the best reference genes for RNAi bioassay. Furthermore, a total of 24 target genes were selected to investigate their RNAi effects in SFB adults with double-stranded RNAs (dsRNAs), five of them showed significant mortality (28.00% to 70.00%), namely Psα-COPI, Psβ-COPI, PsRPS18, Psγ-COPI, and PsArf1COPI. We found that gene transcript levels of the two most lethal genes, Psγ-COPI and PsArf1COPI, were significantly decreased after treated with the target dsRNAs either by feeding or injection method. The findings from this study demonstrated that the introduction of dsRNAs via oral feedings or injection induces the RNAi-mediated silencing of target genes and can lead to insect mortality. Overall, the identified target genes can be explored in developing RNAi-based insecticides for SFB control.

KW - Feeding RNAi

KW - Injection RNAi

KW - Pest control

KW - Phyllotreta striolata

KW - RNA interference

KW - Reference gene

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SN - 0048-3575

VL - 193

JO - Pesticide Biochemistry and Physiology

JF - Pesticide Biochemistry and Physiology

M1 - 105428

ER -

RNAi assays in the striped flea beetle (Phyllotreta striolata) suggest Psγ-COPI and PsArf1COPI as potential molecular targets for pest control

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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