RNAi-based silencing of proteasome 20S subunit alpha 2 affected the survival and development of Henosepilachna vigintioctopunctata

Junna Liu; Mujuan Guo; Satyabrata Nanda; Zhaoyang Li; Xuguo Zhou; Youjun Zhang; Chunxiao Yang; Huipeng Pan

doi:10.1016/j.pestbp.2023.105547

RNAi-based silencing of proteasome 20S subunit alpha 2 affected the survival and development of Henosepilachna vigintioctopunctata

Junna Liu, Mujuan Guo, Satyabrata Nanda, Zhaoyang Li, Xuguo Zhou, Youjun Zhang, Chunxiao Yang, Huipeng Pan

Research output: Contribution to journal › Article › peer-review

Abstract

Henosepilachna vigintioctopunctata is a notorious pest of solanaceous plants in Asia, which is mainly managed by chemical pesticides. RNA interference (RNAi) technique is considered to be a promising and effective alternative for pest control. In this study, we selected the proteasome 20S subunit alpha 2 (Prosα2) gene, a cellular protein involved in many proteins regulatory processes, to explore the RNAi efficiency in H. vigintioctopunctata. The obtained results confirmed the significant lethal effects of HvProsα2 silencing on the H. vigintioctopunctata 1st instar larvae at concentrations of 100, 50, and 5 ng/μL. Ingestion of the bacterially expressed dsHvProsα2 caused high mortality in both larvae and adults. Moreover, silencing of HvProsα2 resulted in feeding disorders, growth delay, and abnormal intestinal development of the larvae. Overall, HvProsα2 acts as an important regulator for the growth and development of H. vigintioctopunctata, and can serve as a candidate target gene for the RNAi-based control of H. vigintioctopunctata.

Original language	English
Article number	105547
Journal	Pesticide Biochemistry and Physiology
Volume	195
DOIs	https://doi.org/10.1016/j.pestbp.2023.105547
State	Published - Sep 2023

Bibliographical note

Publisher Copyright:
© 2023

Funding

This research was supported by the National Natural Science Foundation of China ( 32272628 , 32172500 ). No role has been played by the aforementioned funding agencies in designing, executing, and publishing the study.

Funders	Funder number
National Natural Science Foundation of China (NSFC)	32172500, 32272628

Keywords

Biopesticide
Dietary RNAi
Feeding disorders
Henosepilachna vigintioctopunctata
Prosα2

ASJC Scopus subject areas

Agronomy and Crop Science
Health, Toxicology and Mutagenesis

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10.1016/j.pestbp.2023.105547

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title = "RNAi-based silencing of proteasome 20S subunit alpha 2 affected the survival and development of Henosepilachna vigintioctopunctata",

abstract = "Henosepilachna vigintioctopunctata is a notorious pest of solanaceous plants in Asia, which is mainly managed by chemical pesticides. RNA interference (RNAi) technique is considered to be a promising and effective alternative for pest control. In this study, we selected the proteasome 20S subunit alpha 2 (Prosα2) gene, a cellular protein involved in many proteins regulatory processes, to explore the RNAi efficiency in H. vigintioctopunctata. The obtained results confirmed the significant lethal effects of HvProsα2 silencing on the H. vigintioctopunctata 1st instar larvae at concentrations of 100, 50, and 5 ng/μL. Ingestion of the bacterially expressed dsHvProsα2 caused high mortality in both larvae and adults. Moreover, silencing of HvProsα2 resulted in feeding disorders, growth delay, and abnormal intestinal development of the larvae. Overall, HvProsα2 acts as an important regulator for the growth and development of H. vigintioctopunctata, and can serve as a candidate target gene for the RNAi-based control of H. vigintioctopunctata.",

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author = "Junna Liu and Mujuan Guo and Satyabrata Nanda and Zhaoyang Li and Xuguo Zhou and Youjun Zhang and Chunxiao Yang and Huipeng Pan",

note = "Publisher Copyright: {\textcopyright} 2023",

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doi = "10.1016/j.pestbp.2023.105547",

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AU - Liu, Junna

AU - Guo, Mujuan

AU - Nanda, Satyabrata

AU - Li, Zhaoyang

AU - Zhou, Xuguo

AU - Zhang, Youjun

AU - Yang, Chunxiao

AU - Pan, Huipeng

PY - 2023/9

Y1 - 2023/9

N2 - Henosepilachna vigintioctopunctata is a notorious pest of solanaceous plants in Asia, which is mainly managed by chemical pesticides. RNA interference (RNAi) technique is considered to be a promising and effective alternative for pest control. In this study, we selected the proteasome 20S subunit alpha 2 (Prosα2) gene, a cellular protein involved in many proteins regulatory processes, to explore the RNAi efficiency in H. vigintioctopunctata. The obtained results confirmed the significant lethal effects of HvProsα2 silencing on the H. vigintioctopunctata 1st instar larvae at concentrations of 100, 50, and 5 ng/μL. Ingestion of the bacterially expressed dsHvProsα2 caused high mortality in both larvae and adults. Moreover, silencing of HvProsα2 resulted in feeding disorders, growth delay, and abnormal intestinal development of the larvae. Overall, HvProsα2 acts as an important regulator for the growth and development of H. vigintioctopunctata, and can serve as a candidate target gene for the RNAi-based control of H. vigintioctopunctata.

AB - Henosepilachna vigintioctopunctata is a notorious pest of solanaceous plants in Asia, which is mainly managed by chemical pesticides. RNA interference (RNAi) technique is considered to be a promising and effective alternative for pest control. In this study, we selected the proteasome 20S subunit alpha 2 (Prosα2) gene, a cellular protein involved in many proteins regulatory processes, to explore the RNAi efficiency in H. vigintioctopunctata. The obtained results confirmed the significant lethal effects of HvProsα2 silencing on the H. vigintioctopunctata 1st instar larvae at concentrations of 100, 50, and 5 ng/μL. Ingestion of the bacterially expressed dsHvProsα2 caused high mortality in both larvae and adults. Moreover, silencing of HvProsα2 resulted in feeding disorders, growth delay, and abnormal intestinal development of the larvae. Overall, HvProsα2 acts as an important regulator for the growth and development of H. vigintioctopunctata, and can serve as a candidate target gene for the RNAi-based control of H. vigintioctopunctata.

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KW - Prosα2

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RNAi-based silencing of proteasome 20S subunit alpha 2 affected the survival and development of Henosepilachna vigintioctopunctata

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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