RNAi for management of Asian long-horned beetle, Anoplophora glabripennis: identification of target genes

Ramesh Kumar Dhandapani; Dhandapani Gurusamy; Jian Jun Duan; Subba Reddy Palli

doi:10.1007/s10340-020-01197-8

RNAi for management of Asian long-horned beetle, Anoplophora glabripennis: identification of target genes

Ramesh Kumar Dhandapani, Dhandapani Gurusamy, Jian Jun Duan, Subba Reddy Palli

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

The Asian long-horned beetle (ALB), Anoplophora glabripennis, is a serious invasive forest pest in several countries, including the USA. Currently, there are no effective methods available to manage this pest without adverse impacts on the environment, but RNA interference (RNAi) technology may provide a more environmentally friendly pest control option. To identify target genes for control of ALB, we screened 48 candidate genes in larvae and selected several effective target genes. Injection of double-stranded RNA (dsRNA) targeting the gene coding for the inhibitor of apoptosis (dsIAP) or vacuolar sorting protein, SNF7 (dsSNF7), into larvae and adults resulted in a significant knockdown of these genes and caused 100% mortality in both larvae and adults. As microinjection of dsRNA is not a feasible option for field applications, delivery through oral route was then attempted. Oral delivery of naked dsRNA did not cause mortality in larvae and adults. However, feeding heat-killed bacteria containing dsIAP or dsSNF7 induced mortality and knockdown of target genes in larvae and adults. In conclusion, the utilization of heat-killed bacteria for delivery of dsRNA targeting IAP or SNF7 gene may serve as a potential pest control method for ALB.

Original language	English
Pages (from-to)	823-832
Number of pages	10
Journal	Journal of Pest Science
Volume	93
Issue number	2
DOIs	https://doi.org/10.1007/s10340-020-01197-8
State	Published - Mar 1 2020

Bibliographical note

Funding Information:
This work was funded by USDA Farm Bill Section 10007 (FY2018-AP18PPQS&T00C179) and by the National Institute of Food and Agriculture, USDA, HATCH under 2351177000. Acknowledgements

Funding Information:
We thank Jonathan Schmude and Cherie Keenan (USDA ARS BIIRU) and Jeff Howell of University of Kentucky for assistance with the mortality bioassays with larvae and adults of Asian longhorned beetles, and Linda Saundra, Daria Tatman and Ellen Apacio for help with Asian longhorned beetle rearing at BIIRU.

Publisher Copyright:
© 2020, Springer-Verlag GmbH Germany, part of Springer Nature.

Keywords

Asian long-horned beetle
Double-stranded RNA
Heat-killed bacteria
IAP
SNF7

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Ecology
Agronomy and Crop Science
Plant Science
Insect Science

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10.1007/s10340-020-01197-8

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title = "RNAi for management of Asian long-horned beetle, Anoplophora glabripennis: identification of target genes",

abstract = "The Asian long-horned beetle (ALB), Anoplophora glabripennis, is a serious invasive forest pest in several countries, including the USA. Currently, there are no effective methods available to manage this pest without adverse impacts on the environment, but RNA interference (RNAi) technology may provide a more environmentally friendly pest control option. To identify target genes for control of ALB, we screened 48 candidate genes in larvae and selected several effective target genes. Injection of double-stranded RNA (dsRNA) targeting the gene coding for the inhibitor of apoptosis (dsIAP) or vacuolar sorting protein, SNF7 (dsSNF7), into larvae and adults resulted in a significant knockdown of these genes and caused 100% mortality in both larvae and adults. As microinjection of dsRNA is not a feasible option for field applications, delivery through oral route was then attempted. Oral delivery of naked dsRNA did not cause mortality in larvae and adults. However, feeding heat-killed bacteria containing dsIAP or dsSNF7 induced mortality and knockdown of target genes in larvae and adults. In conclusion, the utilization of heat-killed bacteria for delivery of dsRNA targeting IAP or SNF7 gene may serve as a potential pest control method for ALB.",

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RNAi for management of Asian long-horned beetle, Anoplophora glabripennis: identification of target genes

Abstract

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Keywords

ASJC Scopus subject areas

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