RNAi suppression of the nuclear receptor FTZ-F1 impaired ecdysis, pupation, and reproduction in the 28-spotted potato ladybeetle, Henosepilachna vigintioctopunctata

Zhuoqi Liu, Satyabrata Nanda, Chunxiao Yang, Shimin Chen, Mujuan Guo, Muhammad Musa Khan, Baoli Qiu, Youjun Zhang, Xuguo Zhou, Huipeng Pan

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Fushi-tarazu factor 1 (FTZ–F1) is an ecdysone-inducible transcription factor that plays a vital role during the metamorphosis in insects. In this study, we functionally characterized HvFTZ-F1 in H. vigintioctopunctata, a dreadful solanaceous crop pest, by using a dietary RNA interference technique. The HvFTZ-F1 expression levels were elevated in the 1st and 2nd-instars before molting and declined immediately after ecdysis. The HvFTZ-F1 silencing led to high mortality in the 1st instars, while the expression of the osmosis-regulative gene, HvAQPAn.G, was significantly increased in the 1st instars. HvFTZ-F1 silencing downregulated the Halloween and 20E-related genes, decreased the ecdysteroids titer, suppressed the expression of pigmentation-related genes, and reduced the catecholamines titer. In the 4th instars, HvFTZ-F1 silencing caused 100% mortality by arresting the development at the prepupal stage and preventing new abdominal cuticle formation. In the female adults, HvFTZ-F1 silencing caused an evident decrease in fecundity, prolonged the pre-oviposition period, reduced the number of eggs and hatching rate, severely atrophied the ovaries. Moreover, the 20E-related genes and the dopamine synthesis genes were suppressed in the dsHvFTZ-F1-treated females. Overall, our results revealed that HvFTZ-F1 regulates ecdysis, pupation, and reproduction in H. vigintioctopunctata, thereby could be a promising molecular target for the development of RNAi-based biopesticides to control H. vigintioctopunctata.

Original languageEnglish
Article number105029
JournalPesticide Biochemistry and Physiology
Volume182
DOIs
StatePublished - Mar 2022

Bibliographical note

Funding Information:
This research was supported by the National Natural Science Foundation of China ( 32172500 , 31972269 ) and the National Key R&D Program of China ( 2017YFD0200900 ).

Publisher Copyright:
© 2021

Keywords

  • Ecdysis
  • Henosepilachna vigintioctopunctata
  • HvFTZ-F1
  • Pupation
  • RNA interference
  • Reproduction

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Health, Toxicology and Mutagenesis

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