RNAi turns 25:contributions and challenges in insect science

Subba Reddy Palli

doi:10.3389/finsc.2023.1209478

RNAi turns 25:contributions and challenges in insect science

Subba Reddy Palli

Research output: Contribution to journal › Review article › peer-review

50 Scopus citations

Abstract

Since its discovery in 1998, RNA interference (RNAi), a Nobel prize-winning technology, made significant contributions to advances in biology because of its ability to mediate the knockdown of specific target genes. RNAi applications in medicine and agriculture have been explored with mixed success. The past 25 years of research on RNAi resulted in advances in our understanding of the mechanisms of its action, target specificity, and differential efficiency among animals and plants. RNAi played a major role in advances in insect biology. Did RNAi technology fully meet insect pest and disease vector management expectations? This review will discuss recent advances in the mechanisms of RNAi and its contributions to insect science. The remaining challenges, including delivery to the target site, differential efficiency, potential resistance development and possible solutions for the widespread use of this technology in insect management.

Original language	English
Article number	1209478
Journal	Frontiers in Insect Science
Volume	3
DOIs	https://doi.org/10.3389/finsc.2023.1209478
State	Published - 2023

Bibliographical note

Publisher Copyright:
Copyright © 2023 Palli.

Funding

Research in the Palli laboratory is supported by grants from the National Institutes of Health (GM070559 and 1R21AI131427–01), the National Science Foundation (Industry/University Cooperative Research Centers, the Center for Arthropod Management Technologies under grant IIP-1821936), and the USDA/NIFA (under Hatch Project 2351177000). The content is solely the responsibility of the author and does not necessarily represent the official views of granting agencies.

Funders	Funder number
U.S. Department of Agriculture
Center for Arthropod Management Technologies	IIP-1821936
National Institutes of Health (NIH)	1R21AI131427–01, GM070559
National Science Foundation Arctic Social Science Program	1821936
US Department of Agriculture National Institute of Food and Agriculture, Agriculture and Food Research Initiative	2351177000

Keywords

RNAi
dsRNA
endosomes
insects
nanoparticles
pests

ASJC Scopus subject areas

Insect Science

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10.3389/finsc.2023.1209478

Cite this

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abstract = "Since its discovery in 1998, RNA interference (RNAi), a Nobel prize-winning technology, made significant contributions to advances in biology because of its ability to mediate the knockdown of specific target genes. RNAi applications in medicine and agriculture have been explored with mixed success. The past 25 years of research on RNAi resulted in advances in our understanding of the mechanisms of its action, target specificity, and differential efficiency among animals and plants. RNAi played a major role in advances in insect biology. Did RNAi technology fully meet insect pest and disease vector management expectations? This review will discuss recent advances in the mechanisms of RNAi and its contributions to insect science. The remaining challenges, including delivery to the target site, differential efficiency, potential resistance development and possible solutions for the widespread use of this technology in insect management.",

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AB - Since its discovery in 1998, RNA interference (RNAi), a Nobel prize-winning technology, made significant contributions to advances in biology because of its ability to mediate the knockdown of specific target genes. RNAi applications in medicine and agriculture have been explored with mixed success. The past 25 years of research on RNAi resulted in advances in our understanding of the mechanisms of its action, target specificity, and differential efficiency among animals and plants. RNAi played a major role in advances in insect biology. Did RNAi technology fully meet insect pest and disease vector management expectations? This review will discuss recent advances in the mechanisms of RNAi and its contributions to insect science. The remaining challenges, including delivery to the target site, differential efficiency, potential resistance development and possible solutions for the widespread use of this technology in insect management.

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KW - dsRNA

KW - endosomes

KW - insects

KW - nanoparticles

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DO - 10.3389/finsc.2023.1209478

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VL - 3

JO - Frontiers in Insect Science

JF - Frontiers in Insect Science

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ER -

RNAi turns 25:contributions and challenges in insect science

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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