Role of T-Lymphocytes in the resolution of endotoxin-induced lung injury

An acute neutrophilic lung injury was compared in Balb/c normal and nu/nu (nude) mice to assess the role of T lymphocytes in the resolution of acute pulmonary neutrophilic inflammation following the administration of endotoxin. Maximal neutrophilic infiltration occurred on day 1 post-endotoxin treatment and declined to near normal levels by day 5. In contrast, the percentage of lymphocytes in the bronchoalveolar lavage (BAL) fluid increased from 1.8% on day 1 post-endotoxin to greater than 11% on days three and five, during which time neutrophil resolution was occurring. On days 1-5 after endotoxin administration, approximately 40% of the CD4 lymphocytes expressed the cell surface activation marker, CD69. Despite being CD69⁺, CD4 cells did not express the high affinity IL-2 receptor chain, CD25, to any significant extent on any of the days studied. To assess the contribution of T cells to the rate of clearance of neutrophils from the BAL, normal and nude Balb/c mice were compared for the percentage of neutrophils following nasal administration of endotoxin. Endotoxin-treated nude mice did not demonstrate significant differences in either the total white blood cell counts or in the clearance of neutrophils from the BAL, as compared to normal Balb/c mice. These data indicate that the influx of activated T cells during the resolution of neutrophilic pneumonitis does not contribute to the rate of neutrophil clearance during acute lung injury.