Role of the splenic microenvironment in chronic lymphocytic leukemia development in Eµ-TCL1 transgenic mice

James P. Collard, Mary K. McKenna, Sunil K. Noothi, Sara S. Alhakeem, Jacqueline R. Rivas, Vivek M. Rangnekar, Natarajan Muthusamy, Subbarao Bondada

Research output: Contribution to journalArticlepeer-review

Abstract

The chronic lymphocytic leukemia (CLL) microenvironment has been receiving an increasing amount of attention, but there is currently limited data surrounding how the microenvironment affects initial development of CLL. We determined that the spleen is the initial site of CLL growth through monitoring of transgenic Eμ-TCL1 mice that develop CLL. Subsequently, we isolated stromal cells from the spleens of Eμ-TCL1 mice (EMST cells) that induce CLL cell division in vitro. Both cell–cell contact and soluble factors were involved in EMST-induced CLL cell division. These stromal cells are present in significantly larger numbers in the spleen than other lymphoid organs. We also noted that splenectomy delayed CLL development in Eμ-TCL1 mice and completely prevented CLL development in adoptive transfer mice. Our findings will allow future studies surrounding the CLL microenvironment to focus upon the splenic stromal cells.

Original languageEnglish
Pages (from-to)1810-1822
Number of pages13
JournalLeukemia and Lymphoma
Volume63
Issue number8
DOIs
StatePublished - 2022

Bibliographical note

Publisher Copyright:
© 2022 Informa UK Limited, trading as Taylor & Francis Group.

Keywords

  • Cell lines and animal models
  • basic biology
  • cytokine production and paraneoplastic conditions
  • lymphocytes
  • lymphoid leukemia

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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