TY - JOUR
T1 - Role of the splenic microenvironment in chronic lymphocytic leukemia development in Eµ-TCL1 transgenic mice
AU - Collard, James P.
AU - McKenna, Mary K.
AU - Noothi, Sunil K.
AU - Alhakeem, Sara S.
AU - Rivas, Jacqueline R.
AU - Rangnekar, Vivek M.
AU - Muthusamy, Natarajan
AU - Bondada, Subbarao
N1 - Publisher Copyright:
© 2022 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2022
Y1 - 2022
N2 - The chronic lymphocytic leukemia (CLL) microenvironment has been receiving an increasing amount of attention, but there is currently limited data surrounding how the microenvironment affects initial development of CLL. We determined that the spleen is the initial site of CLL growth through monitoring of transgenic Eμ-TCL1 mice that develop CLL. Subsequently, we isolated stromal cells from the spleens of Eμ-TCL1 mice (EMST cells) that induce CLL cell division in vitro. Both cell–cell contact and soluble factors were involved in EMST-induced CLL cell division. These stromal cells are present in significantly larger numbers in the spleen than other lymphoid organs. We also noted that splenectomy delayed CLL development in Eμ-TCL1 mice and completely prevented CLL development in adoptive transfer mice. Our findings will allow future studies surrounding the CLL microenvironment to focus upon the splenic stromal cells.
AB - The chronic lymphocytic leukemia (CLL) microenvironment has been receiving an increasing amount of attention, but there is currently limited data surrounding how the microenvironment affects initial development of CLL. We determined that the spleen is the initial site of CLL growth through monitoring of transgenic Eμ-TCL1 mice that develop CLL. Subsequently, we isolated stromal cells from the spleens of Eμ-TCL1 mice (EMST cells) that induce CLL cell division in vitro. Both cell–cell contact and soluble factors were involved in EMST-induced CLL cell division. These stromal cells are present in significantly larger numbers in the spleen than other lymphoid organs. We also noted that splenectomy delayed CLL development in Eμ-TCL1 mice and completely prevented CLL development in adoptive transfer mice. Our findings will allow future studies surrounding the CLL microenvironment to focus upon the splenic stromal cells.
KW - Cell lines and animal models
KW - basic biology
KW - cytokine production and paraneoplastic conditions
KW - lymphocytes
KW - lymphoid leukemia
UR - http://www.scopus.com/inward/record.url?scp=85126186976&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85126186976&partnerID=8YFLogxK
U2 - 10.1080/10428194.2022.2045596
DO - 10.1080/10428194.2022.2045596
M3 - Article
C2 - 35258388
AN - SCOPUS:85126186976
SN - 1042-8194
VL - 63
SP - 1810
EP - 1822
JO - Leukemia and Lymphoma
JF - Leukemia and Lymphoma
IS - 8
ER -
