Roles of IL-4 and other factors in trichosanthin-induced ovalbumin-specific IgE response

W. F. Xu, Y. Y. Ji, Y. D. Wu, G. M. Lin, M. Ye, B. Sun

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


AIM: To study the mechanism of trichosanthin (TCS)-induced ovalbumin (OVA)-specific immunoglobulin E (IgE) response in vivo. METHODS: To determine whether interleukin-4 (IL-4) was involved in TCS-induced IgE production, TCS and OVA co-immunized mice were treated with anti-IL-4 monoclonal antibodies (mAb) and OVA-specific serum IgE production was measured by enzyme-linked immunosorbent assay (ELISA). To distinguish whether recombinant IL-4 (rIL-4) was sufficient to support OVA-specific IgE response, OVA alone immunized mice were treated with rIL-4 and OVA-induced IgE production were examined by ELISA in the serum. To determine whether additional factors were involved in TCS-induced IgE response, the kinetic expression of CD40 ligand (CD40L), tumor necrosis factor-α (TNF-α), and interleukin-13 (IL-13) were measured by semi-quantitative RT-PCR in draining mesenteric lymph node (MLN) from TCS-immunized mice. RESULTS: TCS-induced OVA-specific IgE production was suppressed by anti-IL-4 antibody, whereas IL-4 alone could not induce OVA-specific IgE production. CD40L, TNF-α, and IL-13 all expressed high levels in MLN after both primary and secondary immune responses. Among them CD40L had the similar transient expression peak to that of IL-4. CONCLUSION: IL-4 was indispensable for TCS-induced OVA-specific IgE production, and the other three factors examined may also be involved in, but CD40L may play a more important role.

Original languageEnglish
Pages (from-to)736-740
Number of pages5
JournalActa Pharmacologica Sinica
Issue number8
StatePublished - 2001


  • Immunoglobulins
  • Interleukin-13
  • Interleukin-4
  • Ligands
  • Trichosanthin
  • Tumor necrosis factor

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)


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