S. cerevisiae U1 RNA is large and has limited primary sequence homology to metazoan U1 snRNA

Leo Kretzner; Brian C. Rymond; Michael Rosbash

doi:10.1016/0092-8674(87)90032-8

S. cerevisiae U1 RNA is large and has limited primary sequence homology to metazoan U1 snRNA

Leo Kretzner, Brian C. Rymond, Michael Rosbash

Biology

Research output: Contribution to journal › Article › peer-review

85 Scopus citations

Abstract

We have cloned and sequenced the yeast SNR19 gene and show here that snR19 is the yeast homolog of metazoan U1 snRNA. snR19 is 569 nucleotides long, strikingly larger than its metazoan counterpart. The two molecules resemble each other closely in the predicted secondary structure of their first 50 nucleotides. Primary sequence homology is restricted to some of their single-stranded regions, including 11 consecutive nucleotides at the 5′ end of the two molecules, the region that interacts with pre-mRNA 5′ splice junctions. snR19 is spliceosome-associated and required for in vitro pre-mRNA splicing. We also note that 8 sequences in snR19 have extensive complementarity to snR20, the large yeast U2 RNA, suggesting that yeast U1 may interact with yeast U2 by base-pairing.

Original language	English
Pages (from-to)	593-602
Number of pages	10
Journal	Cell
Volume	50
Issue number	4
DOIs	https://doi.org/10.1016/0092-8674(87)90032-8
State	Published - Aug 14 1987

Bibliographical note

Funding Information:
We thank Dana Torrey and Nadja Abovich for their contributions to these experiments, M. Green, C. Pikielny, Y. Citri, H. Colot, B. Seraphin, and P Legrain for comments on the manuscript, and T Tishman for secretarial assistance. This work was supported by National Institutes of Health grant GM23549.

Funding

We thank Dana Torrey and Nadja Abovich for their contributions to these experiments, M. Green, C. Pikielny, Y. Citri, H. Colot, B. Seraphin, and P Legrain for comments on the manuscript, and T Tishman for secretarial assistance. This work was supported by National Institutes of Health grant GM23549.

Funders	Funder number
National Institutes of Health (NIH)
National Institute of General Medical Sciences	R01GM023549

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/0092-8674(87)90032-8

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title = "S. cerevisiae U1 RNA is large and has limited primary sequence homology to metazoan U1 snRNA",

abstract = "We have cloned and sequenced the yeast SNR19 gene and show here that snR19 is the yeast homolog of metazoan U1 snRNA. snR19 is 569 nucleotides long, strikingly larger than its metazoan counterpart. The two molecules resemble each other closely in the predicted secondary structure of their first 50 nucleotides. Primary sequence homology is restricted to some of their single-stranded regions, including 11 consecutive nucleotides at the 5′ end of the two molecules, the region that interacts with pre-mRNA 5′ splice junctions. snR19 is spliceosome-associated and required for in vitro pre-mRNA splicing. We also note that 8 sequences in snR19 have extensive complementarity to snR20, the large yeast U2 RNA, suggesting that yeast U1 may interact with yeast U2 by base-pairing.",

author = "Leo Kretzner and Rymond, {Brian C.} and Michael Rosbash",

note = "Funding Information: We thank Dana Torrey and Nadja Abovich for their contributions to these experiments, M. Green, C. Pikielny, Y. Citri, H. Colot, B. Seraphin, and P Legrain for comments on the manuscript, and T Tishman for secretarial assistance. This work was supported by National Institutes of Health grant GM23549.",

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doi = "10.1016/0092-8674(87)90032-8",

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T1 - S. cerevisiae U1 RNA is large and has limited primary sequence homology to metazoan U1 snRNA

AU - Kretzner, Leo

AU - Rymond, Brian C.

AU - Rosbash, Michael

N1 - Funding Information: We thank Dana Torrey and Nadja Abovich for their contributions to these experiments, M. Green, C. Pikielny, Y. Citri, H. Colot, B. Seraphin, and P Legrain for comments on the manuscript, and T Tishman for secretarial assistance. This work was supported by National Institutes of Health grant GM23549.

PY - 1987/8/14

Y1 - 1987/8/14

N2 - We have cloned and sequenced the yeast SNR19 gene and show here that snR19 is the yeast homolog of metazoan U1 snRNA. snR19 is 569 nucleotides long, strikingly larger than its metazoan counterpart. The two molecules resemble each other closely in the predicted secondary structure of their first 50 nucleotides. Primary sequence homology is restricted to some of their single-stranded regions, including 11 consecutive nucleotides at the 5′ end of the two molecules, the region that interacts with pre-mRNA 5′ splice junctions. snR19 is spliceosome-associated and required for in vitro pre-mRNA splicing. We also note that 8 sequences in snR19 have extensive complementarity to snR20, the large yeast U2 RNA, suggesting that yeast U1 may interact with yeast U2 by base-pairing.

AB - We have cloned and sequenced the yeast SNR19 gene and show here that snR19 is the yeast homolog of metazoan U1 snRNA. snR19 is 569 nucleotides long, strikingly larger than its metazoan counterpart. The two molecules resemble each other closely in the predicted secondary structure of their first 50 nucleotides. Primary sequence homology is restricted to some of their single-stranded regions, including 11 consecutive nucleotides at the 5′ end of the two molecules, the region that interacts with pre-mRNA 5′ splice junctions. snR19 is spliceosome-associated and required for in vitro pre-mRNA splicing. We also note that 8 sequences in snR19 have extensive complementarity to snR20, the large yeast U2 RNA, suggesting that yeast U1 may interact with yeast U2 by base-pairing.

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VL - 50

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JO - Cell

JF - Cell

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S. cerevisiae U1 RNA is large and has limited primary sequence homology to metazoan U1 snRNA

Abstract

Bibliographical note

Funding

ASJC Scopus subject areas

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