We have cloned and sequenced the yeast SNR19 gene and show here that snR19 is the yeast homolog of metazoan U1 snRNA. snR19 is 569 nucleotides long, strikingly larger than its metazoan counterpart. The two molecules resemble each other closely in the predicted secondary structure of their first 50 nucleotides. Primary sequence homology is restricted to some of their single-stranded regions, including 11 consecutive nucleotides at the 5′ end of the two molecules, the region that interacts with pre-mRNA 5′ splice junctions. snR19 is spliceosome-associated and required for in vitro pre-mRNA splicing. We also note that 8 sequences in snR19 have extensive complementarity to snR20, the large yeast U2 RNA, suggesting that yeast U1 may interact with yeast U2 by base-pairing.
|Number of pages
|Published - Aug 14 1987
Bibliographical noteFunding Information:
We thank Dana Torrey and Nadja Abovich for their contributions to these experiments, M. Green, C. Pikielny, Y. Citri, H. Colot, B. Seraphin, and P Legrain for comments on the manuscript, and T Tishman for secretarial assistance. This work was supported by National Institutes of Health grant GM23549.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology (all)