S100β expression in Alzheimer's disease: Relation to neuropathology in brain regions

Linda J. Van Eldik, W. Sue T. Griffin

Research output: Contribution to journalArticlepeer-review

186 Scopus citations

Abstract

S100β levels in tissue homogenates and distribution of S100β-containing activated astrocytes were examined by S100β-specific ELISA and immunohistochemistry, respectively, in brain regions exhibiting many, some, few, or no neuritic plaques in Alzheimer's disease (AD). Compared to samples collected at similar postmortem intervals from control patients of similar ages, S100β levels were elevated in specific brain regions from AD patients, and the overexpression of S100β correlated relatively well with the pattern of regional involvement by neuritic plaques. The largest increases in S100β levels were in hippocampus and temporal lobe, followed by frontal lobe and pons, with no elevation in occipital lobe or cerebellum. Immunohistochemical analysis showed S100β localization primarily in activated astrocytes surrounding neuritic plaques. These results demonstrate that S100β overexpression is brain region-specific and related to astrocyte activation and suggest that elevation of S100β above some threshold is related to the degree of neuropathological involvement of different brain regions in AD.

Original languageEnglish
Pages (from-to)398-403
Number of pages6
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1223
Issue number3
DOIs
StatePublished - Sep 29 1994

Bibliographical note

Funding Information:
These studies were supported in part by NIH grant AG10208 (to L.V.E. and W.S.T.G.). We especially thank Dr. Jin G. Sheng for technical assistance and Dr. Robert E. Mrak for review and comment on the manuscript and Anna Feoktistova, Cynthia Rovnaghi, and Dr. Olcay Jones for assistance in various aspects of this work.

Keywords

  • Alzheimer's disease
  • Cytokine
  • ELISA
  • Glial activation
  • Immunohistochemistry
  • S100β

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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