Salicylic acid inhibits the replication of Tomato bushy stunt virus by directly targeting a host component in the replication complex

Miaoying Tian, Zsuzsanna Sasvari, Paulina Alatriste Gonzalez, Giulia Friso, Elden Rowland, Xiao Min Liu, Klaas J. Van Wijk, Peter D. Nagy, Daniel F. Klessig

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

Although the plant hormone salicylic acid (SA) plays a central role in signaling resistance to viral infection, the underlying mechanisms are only partially understood. Identification and characterization of SA's direct targets have been shown to be an effective strategy for dissecting the complex SA-mediated defense signaling network. In search of additional SA targets, we previously developed two sensitive approaches that utilize SA analogs in conjunction with either a photoaffinity labeling technique or surface plasmon resonance-based technology to identify and evaluate candidate SA-binding proteins (SABPs) from Arabidopsis. Using these approaches, we have now identified several members of the Arabidopsis glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein family, including two chloroplast-localized and two cytosolic isoforms, as SABPs. Cytosolic GAPDH is a well-known glycolytic enzyme; it also is an important host factor involved in the replication of Tomato bushy stunt virus (TBSV), a single-stranded RNA virus. Using a yeast cell-free extract, an in vivo yeast replication system, and plant protoplasts, we demonstrate that SA inhibits TBSV replication. SA does so by inhibiting the binding of cytosolic GAPDH to the negative (2)RNA strand of TBSV. Thus, this study reveals a novel molecular mechanism through which SA regulates virus replication.

Original languageEnglish
Pages (from-to)379-386
Number of pages8
JournalMolecular Plant-Microbe Interactions
Volume28
Issue number4
DOIs
StatePublished - Apr 1 2015

Bibliographical note

Publisher Copyright:
© 2015 The American Phytopathological Society.

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

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