Scavenging of superoxide anion radical by chaparral

Lun Yi Zang; Greg Cosma; Henry Gardner; Kenneth Starks; Xianglin Shi; Val Vallyathan

doi:10.1023/A:1006982523769

Scavenging of superoxide anion radical by chaparral

Lun Yi Zang, Greg Cosma, Henry Gardner, Kenneth Starks, Xianglin Shi, Val Vallyathan

Toxicology and Cancer Biology

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Chaparral is considered to act as an antioxidant. However, the inhibitory effects of chaparral on specific radical species are not well understood. Using electron paramagnetic resonance (EPR) spectroscopy in combination with spin trapping techniques, we have found that chaparral scavenges superoxide anion radical (O₂·^-) in a dose-dependent manner. 5,5-dimethyl-lpyrroline-N-oxide (DMPO) was used as a spin trapping agent and the reaction of xanthine and xanthine oxidase as a source of O₂·^-. The kinetic parameters, IC₅₀ and V(max), for chaparral scavenging of O₂·^- were found to be 0.899 μg/mL and 8.4 ng/mL/sec, respectively. The rate constant for chaparral scavenging O₂·^- was found to be 1.22 x 10⁶ g^-1 s^-1. Our studies suggest that the antioxidant properties of chaparral may involve a direct scavenging effect of the primary oxygen radical, O₂·^-.

Original language	English
Pages (from-to)	157-161
Number of pages	5
Journal	Molecular and Cellular Biochemistry
Volume	196
Issue number	1-2
DOIs	https://doi.org/10.1023/A:1006982523769
State	Published - 1999

Bibliographical note

Funding Information:
This work was supported by US Army Center for Health Research and National Institute for Occupational Safety and Health funding to Colorado State University.

Keywords

Chaparral
EPR
Spin trapping
Superoxide anion radical

ASJC Scopus subject areas

Molecular Biology
Clinical Biochemistry
Cell Biology

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title = "Scavenging of superoxide anion radical by chaparral",

abstract = "Chaparral is considered to act as an antioxidant. However, the inhibitory effects of chaparral on specific radical species are not well understood. Using electron paramagnetic resonance (EPR) spectroscopy in combination with spin trapping techniques, we have found that chaparral scavenges superoxide anion radical (O2·-) in a dose-dependent manner. 5,5-dimethyl-lpyrroline-N-oxide (DMPO) was used as a spin trapping agent and the reaction of xanthine and xanthine oxidase as a source of O2·-. The kinetic parameters, IC50 and V(max), for chaparral scavenging of O2·- were found to be 0.899 μg/mL and 8.4 ng/mL/sec, respectively. The rate constant for chaparral scavenging O2·- was found to be 1.22 x 106 g-1 s-1. Our studies suggest that the antioxidant properties of chaparral may involve a direct scavenging effect of the primary oxygen radical, O2·-.",

keywords = "Chaparral, EPR, Spin trapping, Superoxide anion radical",

author = "Zang, {Lun Yi} and Greg Cosma and Henry Gardner and Kenneth Starks and Xianglin Shi and Val Vallyathan",

note = "Funding Information: This work was supported by US Army Center for Health Research and National Institute for Occupational Safety and Health funding to Colorado State University.",

year = "1999",

doi = "10.1023/A:1006982523769",

language = "English",

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pages = "157--161",

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T1 - Scavenging of superoxide anion radical by chaparral

AU - Zang, Lun Yi

AU - Cosma, Greg

AU - Gardner, Henry

AU - Starks, Kenneth

AU - Shi, Xianglin

AU - Vallyathan, Val

N1 - Funding Information: This work was supported by US Army Center for Health Research and National Institute for Occupational Safety and Health funding to Colorado State University.

PY - 1999

Y1 - 1999

N2 - Chaparral is considered to act as an antioxidant. However, the inhibitory effects of chaparral on specific radical species are not well understood. Using electron paramagnetic resonance (EPR) spectroscopy in combination with spin trapping techniques, we have found that chaparral scavenges superoxide anion radical (O2·-) in a dose-dependent manner. 5,5-dimethyl-lpyrroline-N-oxide (DMPO) was used as a spin trapping agent and the reaction of xanthine and xanthine oxidase as a source of O2·-. The kinetic parameters, IC50 and V(max), for chaparral scavenging of O2·- were found to be 0.899 μg/mL and 8.4 ng/mL/sec, respectively. The rate constant for chaparral scavenging O2·- was found to be 1.22 x 106 g-1 s-1. Our studies suggest that the antioxidant properties of chaparral may involve a direct scavenging effect of the primary oxygen radical, O2·-.

AB - Chaparral is considered to act as an antioxidant. However, the inhibitory effects of chaparral on specific radical species are not well understood. Using electron paramagnetic resonance (EPR) spectroscopy in combination with spin trapping techniques, we have found that chaparral scavenges superoxide anion radical (O2·-) in a dose-dependent manner. 5,5-dimethyl-lpyrroline-N-oxide (DMPO) was used as a spin trapping agent and the reaction of xanthine and xanthine oxidase as a source of O2·-. The kinetic parameters, IC50 and V(max), for chaparral scavenging of O2·- were found to be 0.899 μg/mL and 8.4 ng/mL/sec, respectively. The rate constant for chaparral scavenging O2·- was found to be 1.22 x 106 g-1 s-1. Our studies suggest that the antioxidant properties of chaparral may involve a direct scavenging effect of the primary oxygen radical, O2·-.

KW - Chaparral

KW - EPR

KW - Spin trapping

KW - Superoxide anion radical

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AN - SCOPUS:0032769003

SN - 0300-8177

VL - 196

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EP - 161

JO - Molecular and Cellular Biochemistry

JF - Molecular and Cellular Biochemistry

IS - 1-2

ER -

Scavenging of superoxide anion radical by chaparral

Abstract

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Keywords

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