Searching for hemostatic modifier genes affecting the phenotype of mice with very low levels of FVII

Elliot D. Rosen, Xiaoling Xuei, Mark Suckow, Howard Edenberg

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

FVIItTA/- mice are heterozygous for the FVII- null allele and a gene-targeted allele expressing very low levels of FVII. Approximately half the FVIItTA/- in a mixed C57Bl/6:129x1/SVJ background survive much longer than mice in a C57Bl/6 background, suggesting that genetic background affects the survival of mice expressing very low levels of FVII. A lineage of long-term surviving FVIItTA/- mice in a predominantly C57Bl/6 background was generated by selecting 16 weeks or older FVIItTA/- males and breeding them with C57Bl/6 FVII+/- females. It is postulated that these mice maintain 129x1/SVJ alleles at putative hemostatic modifier loci necessary for the survival of mice expressing very low levels of FVII. A SNP genomic scan was performed on DNA from long-term surviving FVIItTA/- mice. The scan identified 8 regions enriched for 129x1/SVJ sequences that might contain putative hemostatic modifier genes.

Original languageEnglish
Pages (from-to)131-134
Number of pages4
JournalBlood Cells, Molecules, and Diseases
Volume36
Issue number2
DOIs
StatePublished - Mar 2006

Bibliographical note

Funding Information:
The authors wish to acknowledge Valerie Schroeder for her management of the mouse colonies. The work was supported by NIH grants P01 HL73750 and HL65241A to EDR.

Funding

The authors wish to acknowledge Valerie Schroeder for her management of the mouse colonies. The work was supported by NIH grants P01 HL73750 and HL65241A to EDR.

FundersFunder number
National Institutes of Health (NIH)P01 HL73750
National Heart, Lung, and Blood Institute (NHLBI)R01HL065241

    Keywords

    • Factor VII
    • Hemostasis
    • Modifier genes
    • Quantitative traits
    • SNPs
    • Strain differences

    ASJC Scopus subject areas

    • Molecular Medicine
    • Molecular Biology
    • Hematology
    • Cell Biology

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