Abstract
FVIItTA/- mice are heterozygous for the FVII- null allele and a gene-targeted allele expressing very low levels of FVII. Approximately half the FVIItTA/- in a mixed C57Bl/6:129x1/SVJ background survive much longer than mice in a C57Bl/6 background, suggesting that genetic background affects the survival of mice expressing very low levels of FVII. A lineage of long-term surviving FVIItTA/- mice in a predominantly C57Bl/6 background was generated by selecting 16 weeks or older FVIItTA/- males and breeding them with C57Bl/6 FVII+/- females. It is postulated that these mice maintain 129x1/SVJ alleles at putative hemostatic modifier loci necessary for the survival of mice expressing very low levels of FVII. A SNP genomic scan was performed on DNA from long-term surviving FVIItTA/- mice. The scan identified 8 regions enriched for 129x1/SVJ sequences that might contain putative hemostatic modifier genes.
Original language | English |
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Pages (from-to) | 131-134 |
Number of pages | 4 |
Journal | Blood Cells, Molecules, and Diseases |
Volume | 36 |
Issue number | 2 |
DOIs | |
State | Published - Mar 2006 |
Bibliographical note
Funding Information:The authors wish to acknowledge Valerie Schroeder for her management of the mouse colonies. The work was supported by NIH grants P01 HL73750 and HL65241A to EDR.
Funding
The authors wish to acknowledge Valerie Schroeder for her management of the mouse colonies. The work was supported by NIH grants P01 HL73750 and HL65241A to EDR.
Funders | Funder number |
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National Institutes of Health (NIH) | P01 HL73750 |
National Heart, Lung, and Blood Institute (NHLBI) | R01HL065241 |
Keywords
- Factor VII
- Hemostasis
- Modifier genes
- Quantitative traits
- SNPs
- Strain differences
ASJC Scopus subject areas
- Molecular Medicine
- Molecular Biology
- Hematology
- Cell Biology