Secretion of insulin degrading enzyme by Jurkat T -cells

A. Safavi, L. Cottam, L. B. Hersh

Research output: Contribution to journalArticlepeer-review


We have previously observed secretion of insulin degrading enzyme (IDE, B.C. from the EL-4 Tcell line and anti-CD3 activated murine CD4 + T-cells under serum starved conditions. We now show that IDE is secreted from Jurkat T-cells under normal growth conditions. The molecular properties of the secreted IDE suggest that its secretion does not involve processing nor is the enzyme glycosylated. In addition to Jurkat T-cells, we have observed IDE secretion by the MO 1094 B-cell lines, but not by a number of IDE containing, non-lymphocyte cell lines. Thus, it seems that IDE secretion is specific to B and T-cells. It appears that lymphocytes have a unique secretion mechanism for IDE. The secreted IDE might represent a new mechanism for regulating the extracellular concentration of peptides.

Original languageEnglish
Pages (from-to)A1404
JournalFASEB Journal
Issue number6
StatePublished - 1996

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics


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