Abstract
We have previously observed secretion of insulin degrading enzyme (IDE, B.C. 3.4.24.55) from the EL-4 Tcell line and anti-CD3 activated murine CD4 + T-cells under serum starved conditions. We now show that IDE is secreted from Jurkat T-cells under normal growth conditions. The molecular properties of the secreted IDE suggest that its secretion does not involve processing nor is the enzyme glycosylated. In addition to Jurkat T-cells, we have observed IDE secretion by the MO 1094 B-cell lines, but not by a number of IDE containing, non-lymphocyte cell lines. Thus, it seems that IDE secretion is specific to B and T-cells. It appears that lymphocytes have a unique secretion mechanism for IDE. The secreted IDE might represent a new mechanism for regulating the extracellular concentration of peptides.
| Original language | English |
|---|---|
| Pages (from-to) | A1404 |
| Journal | FASEB Journal |
| Volume | 10 |
| Issue number | 6 |
| State | Published - 1996 |
ASJC Scopus subject areas
- Biotechnology
- Biochemistry
- Molecular Biology
- Genetics