Secretion of progesterone, estradiol-17β, PGE, PGF(2α), and pregnancy- specific protein B by 90-day intact and ovariectomized pregnant ewes

Y. S. Weems, P. J. Bridges, B. R. Leamaster, R. G. Sasser, D. L. Vincent, C. W. Weems

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Ninety-day pregnant ewes were either laparotomized, ovaries left in situ or bilaterally ovariectomized, and a jugular venous catheter and an inferior vena cava catheter via the saphenous vein were installed. Seven days later, placenta slices were collected and incubated in vitro for 4 h. Secretions of progesterone, PGE, estradiol-17β and pregnancy-specific protein B (PSPB) in vitro by placenta from ovariectomized ewes were increased (P ≤ 0.05) by 2.7- , 3.6-, 2.2-, and 2.4-fold, respectively, when compared to placenta slices from intact 90-day pregnant ewes. Secretion of PGF(2α) in vitro was unchanged (P ≥ 0.05). Ovariectomy decreased (P ≤ 0.05) jugular venous progesterone for 78 h followed by a quadratic increase (P ≤ 0.05), whereas progesterone remained unchanged (P ≥ 0.05) in intact ewes over the 162-h sampling period. Ovariectomy increased (P ≤ 0.05) PGE in inferior vena cava plasma over the last half of the 162-h sampling period, whereas concentration of PGF(2α) did not change (P ≥ 0.05). Increases in PGE occurred before the increase in progesterone. Concentrations of PSPB in inferior vena cava plasma of ovariectomized pregnant ewes increased (P ≤ 0.05) during the last half of the 162-h sampling period, but not in intact ewes (P ≥ 0.05). PSPB increased before PGE and progesterone. Concentrations of estradiol-17β in jugular venous plasma of ovariectomized pregnant ewes increased (P ≤ 0.05) during the last half of the sampling period, but not in intact ewes (P ≥ 0.05). Increases in estradiol-17β occurred before increases in PSPB. It is concluded that these data support the hypothesis that estradiol-17β may control placental secretion of PSPB; PSPB may regulate placental secretion of PGE; and PGE may regulate placental secretion of progesterone.

Original languageEnglish
Pages (from-to)139-148
Number of pages10
JournalProstaglandins and Other Lipid Mediators
Volume58
Issue number2-4
DOIs
StatePublished - Oct 1999

Bibliographical note

Funding Information:
☆ This research was supported in part by USDA–CSREES Special Grant No. 95-34135-1776, to C.W. Weems and managed by the Pacific Basin Advisory Group and Hawaii Hatch Project 259 as its contribution to USDA W–112 Regional Research Project.

Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.

Keywords

  • Estradiol-17β
  • PGE
  • PGF(2α)
  • Placenta
  • Pregnancy- specific protein B
  • Progesterone
  • Sheep

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Pharmacology
  • Cell Biology

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