Secretion of S-100 from rat C6 glioma cells

Linda J. Van Eldik, Danna B. Zimmer

Research output: Contribution to journalArticlepeer-review

155 Scopus citations


Conditioned media from rat C6 glioma cells contain significant levels of S-100 immunoreactivity as determined by radioimmunoassay. The levels of extracellular S-100 detected could not be accounted for by the release of intracellular S-100 into the media from lysed cells. The extracellular form of S-100 exhibits fractionation properties and immunological characteristics that are different from those of the intracellular form of S-100 in C6 cells. While the intracellular S-100 levels increase as a function of days in culture, the extracellular S-100 levels are high until the cells reach confluency and are lower in postconfluent cultures. Altogether, our data suggest that C6 glioma cells secrete S-100, and that the quantitative levels of the intracellular and secreted forms of S-100 are differentially regulated.

Original languageEnglish
Pages (from-to)367-370
Number of pages4
JournalBrain Research
Issue number2
StatePublished - Dec 15 1987

Bibliographical note

Funding Information:
We thank Jay Hodge and RebeccaM iller for careful and competenta ssistancew ith these studies. These studiesw eres upportedin part by National In-stituteso f Health Grant GM-33481.


  • Radioimmunoassay
  • Rat glioma cell
  • S-100
  • Secretion

ASJC Scopus subject areas

  • Neuroscience (all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology


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