A binary plasmid was constructed to contain the mouse metallothionein c-DNA, the constitutive 35S promoter from cauliflower mosaic virus, the polyadenylation signal from the pea rbcS-E9 gene and several selectable markers. The plasmid was transferred to Agrobacterium tumefaciens and the leaf disc method was used to transform tobacco. Callus and shoots were regenerated in the presence of kanamycin and transformed plants were obtained. Southern, Northern and Western blot analysis demonstrated integration and expression of the metallothionein gene in transformed callus and transgenic plants. The gene is transmitted to and expressed in seed derived progeny as a dominant Mendelian trait.
|Number of pages||8|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Jan 29 1988|
Copyright 2014 Elsevier B.V., All rights reserved.
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology