Seedling tolerance to cotyledon removal varies with seed size: A case of five legume species

Xiao Wen Hu, Rui Zhang, Yan Pei Wu, Carol C. Baskin

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


It is generally accepted that seedlings from large seeds are more tolerant to defoliation than those from small seeds due to the additional metabolic reserves present in the large seeds. However, information on the effects of amount of seed reserves (cotyledon removal) from seedlings resulting from large vs. small seeds on seedling growth and long-term survival in the field is limited. Five legume species with different sizes of seeds were sown in the field and none, one, or both cotyledons removed 7 days after seedling emergence. Seedling biomass, relative growth rate (RGR) and survival were determined at different time. Cotyledon removal, species, and their interaction had significant effects on seedling growth and survival. During the period between 33 and 70 days, seedlings from large seeds had a significantly lower RGR than those from small seeds. Biomass, RGR, and survival of seedlings from large seeds were significantly reduced by removal one or both cotyledons, whereas those of seedlings from small seeds were not affected. Seed energy reserves are more important for the early growth of seedlings from large seeds than for those from small seeds. The overall effect of cotyledon removal on growth and survival varies with seed size (i.e., energy reserves) with seedlings from small seeds being less sensitive than those from large seeds under field conditions.

Original languageEnglish
Pages (from-to)5948-5955
Number of pages8
JournalEcology and Evolution
Issue number15
StatePublished - Aug 2017

Bibliographical note

Publisher Copyright:
© 2017 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.


  • cotyledon damage
  • relative growth rate
  • seed mass
  • seedling survival

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Nature and Landscape Conservation


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