A method to selectively spin label galactose and N-acetylgalactosamine residues of erythrocyte membrane glycoconjugates is described. The method is based on the activation of the C-6 CH2OH group of these two sugars by galactose oxidase followed by reductive amination with 2,2,6,6-tetramethyl-4-aminopiperidine-1-oxyl in the presence of a mild reducing agent, NaBH3CN. The extent and distribution of the spin labeling suggest that the major sialoglycoprotein, PAS-1, incorporates the greatest amount of spin label while the glycolipids incorporate less than `0% of the spin label.
|Number of pages
|Journal of Biochemical and Biophysical Methods
|Published - Nov 1984
Bibliographical noteFunding Information:
This work was supportedin part by NIH grants AG-00084 and AG-02759 and a grant from the Alzheimer'sD iseaseand Related Disorders Association,In c.
- cell surface carbohydrates
- erythrocytes membrane
- glycophorin A
- selectivity for galactose residues
- spin labeling
ASJC Scopus subject areas