Selective spin labeling of terminal galactose and N-acetylgalactosamine residues on the membrane surface of erythrocytes

Bennett T. Farmer; D. Allan Butterfield

doi:10.1016/0165-022X(84)90057-5

Selective spin labeling of terminal galactose and N-acetylgalactosamine residues on the membrane surface of erythrocytes

Bennett T. Farmer, D. Allan Butterfield

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A method to selectively spin label galactose and N-acetylgalactosamine residues of erythrocyte membrane glycoconjugates is described. The method is based on the activation of the C-6 CH₂OH group of these two sugars by galactose oxidase followed by reductive amination with 2,2,6,6-tetramethyl-4-aminopiperidine-1-oxyl in the presence of a mild reducing agent, NaBH₃CN. The extent and distribution of the spin labeling suggest that the major sialoglycoprotein, PAS-1, incorporates the greatest amount of spin label while the glycolipids incorporate less than `0% of the spin label.

Original language	English
Pages (from-to)	111-120
Number of pages	10
Journal	Journal of Biochemical and Biophysical Methods
Volume	10
Issue number	1-2
DOIs	https://doi.org/10.1016/0165-022X(84)90057-5
State	Published - Nov 1984

Bibliographical note

Funding Information:
This work was supportedin part by NIH grants AG-00084 and AG-02759 and a grant from the Alzheimer'sD iseaseand Related Disorders Association,In c.

Funding

This work was supportedin part by NIH grants AG-00084 and AG-02759 and a grant from the Alzheimer'sD iseaseand Related Disorders Association,In c.

Funders	Funder number
Alzheimer'sD iseaseand Related Disorders Association
National Institutes of Health (NIH)	AG-00084, AG-02759

Keywords

ESR
cell surface carbohydrates
erythrocytes membrane
glycophorin A
selectivity for galactose residues
spin labeling

ASJC Scopus subject areas

Biophysics
Biochemistry

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10.1016/0165-022X(84)90057-5

Cite this

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title = "Selective spin labeling of terminal galactose and N-acetylgalactosamine residues on the membrane surface of erythrocytes",

abstract = "A method to selectively spin label galactose and N-acetylgalactosamine residues of erythrocyte membrane glycoconjugates is described. The method is based on the activation of the C-6 CH2OH group of these two sugars by galactose oxidase followed by reductive amination with 2,2,6,6-tetramethyl-4-aminopiperidine-1-oxyl in the presence of a mild reducing agent, NaBH3CN. The extent and distribution of the spin labeling suggest that the major sialoglycoprotein, PAS-1, incorporates the greatest amount of spin label while the glycolipids incorporate less than `0\% of the spin label.",

keywords = "ESR, cell surface carbohydrates, erythrocytes membrane, glycophorin A, selectivity for galactose residues, spin labeling",

author = "Farmer, \{Bennett T.\} and Butterfield, \{D. Allan\}",

note = "Funding Information: This work was supportedin part by NIH grants AG-00084 and AG-02759 and a grant from the Alzheimer'sD iseaseand Related Disorders Association,In c.",

year = "1984",

month = nov,

doi = "10.1016/0165-022X(84)90057-5",

language = "English",

volume = "10",

pages = "111--120",

number = "1-2",

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TY - JOUR

T1 - Selective spin labeling of terminal galactose and N-acetylgalactosamine residues on the membrane surface of erythrocytes

AU - Farmer, Bennett T.

AU - Butterfield, D. Allan

N1 - Funding Information: This work was supportedin part by NIH grants AG-00084 and AG-02759 and a grant from the Alzheimer'sD iseaseand Related Disorders Association,In c.

PY - 1984/11

Y1 - 1984/11

N2 - A method to selectively spin label galactose and N-acetylgalactosamine residues of erythrocyte membrane glycoconjugates is described. The method is based on the activation of the C-6 CH2OH group of these two sugars by galactose oxidase followed by reductive amination with 2,2,6,6-tetramethyl-4-aminopiperidine-1-oxyl in the presence of a mild reducing agent, NaBH3CN. The extent and distribution of the spin labeling suggest that the major sialoglycoprotein, PAS-1, incorporates the greatest amount of spin label while the glycolipids incorporate less than `0% of the spin label.

AB - A method to selectively spin label galactose and N-acetylgalactosamine residues of erythrocyte membrane glycoconjugates is described. The method is based on the activation of the C-6 CH2OH group of these two sugars by galactose oxidase followed by reductive amination with 2,2,6,6-tetramethyl-4-aminopiperidine-1-oxyl in the presence of a mild reducing agent, NaBH3CN. The extent and distribution of the spin labeling suggest that the major sialoglycoprotein, PAS-1, incorporates the greatest amount of spin label while the glycolipids incorporate less than `0% of the spin label.

KW - ESR

KW - cell surface carbohydrates

KW - erythrocytes membrane

KW - glycophorin A

KW - selectivity for galactose residues

KW - spin labeling

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AN - SCOPUS:0021722510

SN - 0165-022X

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EP - 120

JO - Journal of Biochemical and Biophysical Methods

JF - Journal of Biochemical and Biophysical Methods

IS - 1-2

ER -

Selective spin labeling of terminal galactose and N-acetylgalactosamine residues on the membrane surface of erythrocytes

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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