The glutamine in position 69 is one of only three conserved active-site amino acid differences between Fe- and Mn-containing superoxide dismutases (SODs). We have refined the conditions for extremely selective labeling of the side chains of glutamine with 15N, and thus obtained dramatically simplified spectra, despite the large size of Fe-SOD. The improved resolution afforded by such highly specific labeling permits the use of direct 15N detection to observe and assign Gln 69, even though its distance to the paramagnetic Fe2+ is only 5 Å. Selective glutamine side-chain labeling is inexpensive and has general utility for large (and paramagnet-containing) proteins.
|Number of pages||6|
|Journal||Journal of Biomolecular NMR|
|State||Published - 1997|
Bibliographical noteFunding Information:
This work was supported by N.S.F. grant MCB-9418181, the Petroleum Research Foundation ACS-PRF 28379-G4, and an institutional research grant from the American Cancer Society, IRG 11-33. C.K.V. is supported by NIGMS/N.I.H. training grant T32-GM07231.
Copyright 2018 Elsevier B.V., All rights reserved.
- Selective labeling
- Superoxide dismutase
ASJC Scopus subject areas