TY - JOUR
T1 - Sensitivity of immunohistochemistry and polymerase chain reaction in detecting prostate cancer cells in bone marrow
AU - Wood, D. P.
AU - Banks, E. R.
AU - Humphreys, S.
AU - Rangnekar, V. M.
PY - 1994
Y1 - 1994
N2 - Occult micrometastases detected by immunohistochemistry have prognostic significance in patients with localized breast cancer. To determine the usefulness of this technique and of polymerase chain reaction in detecting occult prostate cancer, we evaluated the sensitivity and specificity of immunohistochemistry and polymerase chain reaction amplification of mRNA to detect prostate cancer cells in bone marrow samples. We used cells from an established prostate cancer cell line (LNCAP) mixed with lymphocytes at various dilutions from 105 cancer cells in 106 lymphocytes to 1:106. Both techniques had a 100% specificity and identified cancer cells at all dilutions. Polymerase chain reaction was more sensitive than immunohistochemistry at the lowest dilutions (10-5 and 10-6, p = 0.033). We have evaluated seven patients with prostate cancer for micrometastases. Both of the patients with known metastatic prostate cancer and one of the five patients with clinically localized tumors had micrometastases. Detection of micrometastases may be useful in the staging of prostate cancer.
AB - Occult micrometastases detected by immunohistochemistry have prognostic significance in patients with localized breast cancer. To determine the usefulness of this technique and of polymerase chain reaction in detecting occult prostate cancer, we evaluated the sensitivity and specificity of immunohistochemistry and polymerase chain reaction amplification of mRNA to detect prostate cancer cells in bone marrow samples. We used cells from an established prostate cancer cell line (LNCAP) mixed with lymphocytes at various dilutions from 105 cancer cells in 106 lymphocytes to 1:106. Both techniques had a 100% specificity and identified cancer cells at all dilutions. Polymerase chain reaction was more sensitive than immunohistochemistry at the lowest dilutions (10-5 and 10-6, p = 0.033). We have evaluated seven patients with prostate cancer for micrometastases. Both of the patients with known metastatic prostate cancer and one of the five patients with clinically localized tumors had micrometastases. Detection of micrometastases may be useful in the staging of prostate cancer.
KW - Immunohistochemistry
KW - Polymerase chain reaction
KW - Prostate neoplasms
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U2 - 10.1177/42.4.7510319
DO - 10.1177/42.4.7510319
M3 - Article
C2 - 7510319
AN - SCOPUS:0028345354
SN - 0022-1554
VL - 42
SP - 505
EP - 511
JO - Journal of Histochemistry and Cytochemistry
JF - Journal of Histochemistry and Cytochemistry
IS - 4
ER -