TY - JOUR
T1 - Sensitivity of the Causal Agent of Northern Leaf Blight of Corn, Exserohilum turcicum, to the Demethylase-Inhibiting Fungicide Flutriafol
AU - Anderson, Nolan R.
AU - McCoy, Austin G.
AU - Castellano, Cristina
AU - Chilvers, Martin I.
AU - Alinger, Eric
AU - Allen, Tom W.
AU - Bissonnette, Kaitlyn
AU - Copeland, Drake
AU - Hustedde, Nick
AU - Jackson-Ziems, Tamra A.
AU - Kleczewski, Nathan
AU - Leon, Christopher
AU - Mueller, John
AU - Price, Paul
AU - Raid, Richard
AU - Robertson, Alison E.
AU - Sikora, Edward J.
AU - Telenko, Darcy E.P.
AU - Wiggins, Matthew
AU - Wise, Kiersten A.
N1 - Publisher Copyright:
© 2024 The American Phytopathological Society
PY - 2024
Y1 - 2024
N2 - Fungicides including active ingredients in the demethylation inhibitor group (Fungicide Resistance Action Committee Group 3) are used to manage corn diseases such as northern corn leaf blight, caused by Exserohilum turcicum. This study assessed the level of sensitivity to the demethylation-inhibiting fungicide flutriafol on a population of 81 E. turcicum isolates collected from across the United States in 2020. Sensitivity to flutriafol for E. turcicum was assessed using fungicide-amended media to determine the effective concentration needed to limit hyphal growth by 50%. The mean absolute EC50 values of the overall population calculated using the four-parameter log-logistic and Weibull2 four-parameter models were 2.1881 and 3.2643 μg/ml, respectively. The growth of most isolates (n = 70, 86.4%) was completely inhibited by 1 μg/ml of flutriafol. However, the growth of seven isolates (8.6%) was only completely inhibited at 10 or 100 μg/ml, and complete inhibition was not observed at 100 μg/ml for four isolates (4.9%). These results suggest that there may be isolates of E. turcicum in the United States that are resistant to flutriafol. Additional fungicide sensitivity screening is critical to monitor the development of fungicide resistance in U.S. populations of E. turcicum.
AB - Fungicides including active ingredients in the demethylation inhibitor group (Fungicide Resistance Action Committee Group 3) are used to manage corn diseases such as northern corn leaf blight, caused by Exserohilum turcicum. This study assessed the level of sensitivity to the demethylation-inhibiting fungicide flutriafol on a population of 81 E. turcicum isolates collected from across the United States in 2020. Sensitivity to flutriafol for E. turcicum was assessed using fungicide-amended media to determine the effective concentration needed to limit hyphal growth by 50%. The mean absolute EC50 values of the overall population calculated using the four-parameter log-logistic and Weibull2 four-parameter models were 2.1881 and 3.2643 μg/ml, respectively. The growth of most isolates (n = 70, 86.4%) was completely inhibited by 1 μg/ml of flutriafol. However, the growth of seven isolates (8.6%) was only completely inhibited at 10 or 100 μg/ml, and complete inhibition was not observed at 100 μg/ml for four isolates (4.9%). These results suggest that there may be isolates of E. turcicum in the United States that are resistant to flutriafol. Additional fungicide sensitivity screening is critical to monitor the development of fungicide resistance in U.S. populations of E. turcicum.
KW - flutriafol
KW - fungicide sensitivity
KW - northern corn leaf blight
KW - triazole
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U2 - 10.1094/PHP-11-23-0098-RS
DO - 10.1094/PHP-11-23-0098-RS
M3 - Article
AN - SCOPUS:85201444626
SN - 1535-1025
VL - 25
SP - 287
EP - 292
JO - Plant Health Progress
JF - Plant Health Progress
IS - 3
ER -