Two methods have been developed to separate plant extract water-soluble proteins from secondary metabolites, in particular from plant phenolic compounds. The first method is chromatography of a plant extract on Toyopearl HW60 in ammonium sulfate solution. The second is differential precipitation of proteins and phenolic compounds with ammonium sulfate in the presence of polyethylene glycol at low concentrations. Both methods were used for isolation of cytokinin-binding protein with a molecular mass of 70 kDa from maize (Zea mays) coleoptiles and gave good results. These techniques can be used for isolation of any water-soluble protein from plants.
|Number of pages||5|
|Journal||Plant Physiology and Biochemistry|
|State||Published - Oct 1998|
Bibliographical noteFunding Information:
kind help in the preparation of the manuscript. This work was supported by the Russian Fund for Basic Research (Grant 9504-12005, 96-15-98030) and by INTAS (Grant 93-678).
- Ammonium sulfate precipitation
- Hydrophobic interaction chromatography
- Phenol separation
- Polyethylene glycol fractionation
- Protein isolation
ASJC Scopus subject areas
- Plant Science