Separation of proteins from phenols in cereal leaf extract by hydrophobic interaction- ammonium sulfate fractionation

Feodor A. Brovko; Tatiana K. Zagranichnaya

doi:10.1016/S0981-9428(98)80029-6

Separation of proteins from phenols in cereal leaf extract by hydrophobic interaction- ammonium sulfate fractionation

Feodor A. Brovko, Tatiana K. Zagranichnaya

Internal Medicine

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9 Scopus citations

Abstract

Two methods have been developed to separate plant extract water-soluble proteins from secondary metabolites, in particular from plant phenolic compounds. The first method is chromatography of a plant extract on Toyopearl HW60 in ammonium sulfate solution. The second is differential precipitation of proteins and phenolic compounds with ammonium sulfate in the presence of polyethylene glycol at low concentrations. Both methods were used for isolation of cytokinin-binding protein with a molecular mass of 70 kDa from maize (Zea mays) coleoptiles and gave good results. These techniques can be used for isolation of any water-soluble protein from plants.

Original language	English
Pages (from-to)	773-777
Number of pages	5
Journal	Plant Physiology and Biochemistry
Volume	36
Issue number	10
DOIs	https://doi.org/10.1016/S0981-9428(98)80029-6
State	Published - Oct 1998

Bibliographical note

Funding Information:
kind help in the preparation of the manuscript. This work was supported by the Russian Fund for Basic Research (Grant 9504-12005, 96-15-98030) and by INTAS (Grant 93-678).

Keywords

Ammonium sulfate precipitation
Hydrophobic interaction chromatography
Phenol separation
Polyethylene glycol fractionation
Protein isolation

ASJC Scopus subject areas

Physiology
Genetics
Plant Science

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10.1016/S0981-9428(98)80029-6

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title = "Separation of proteins from phenols in cereal leaf extract by hydrophobic interaction- ammonium sulfate fractionation",

abstract = "Two methods have been developed to separate plant extract water-soluble proteins from secondary metabolites, in particular from plant phenolic compounds. The first method is chromatography of a plant extract on Toyopearl HW60 in ammonium sulfate solution. The second is differential precipitation of proteins and phenolic compounds with ammonium sulfate in the presence of polyethylene glycol at low concentrations. Both methods were used for isolation of cytokinin-binding protein with a molecular mass of 70 kDa from maize (Zea mays) coleoptiles and gave good results. These techniques can be used for isolation of any water-soluble protein from plants.",

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N2 - Two methods have been developed to separate plant extract water-soluble proteins from secondary metabolites, in particular from plant phenolic compounds. The first method is chromatography of a plant extract on Toyopearl HW60 in ammonium sulfate solution. The second is differential precipitation of proteins and phenolic compounds with ammonium sulfate in the presence of polyethylene glycol at low concentrations. Both methods were used for isolation of cytokinin-binding protein with a molecular mass of 70 kDa from maize (Zea mays) coleoptiles and gave good results. These techniques can be used for isolation of any water-soluble protein from plants.

AB - Two methods have been developed to separate plant extract water-soluble proteins from secondary metabolites, in particular from plant phenolic compounds. The first method is chromatography of a plant extract on Toyopearl HW60 in ammonium sulfate solution. The second is differential precipitation of proteins and phenolic compounds with ammonium sulfate in the presence of polyethylene glycol at low concentrations. Both methods were used for isolation of cytokinin-binding protein with a molecular mass of 70 kDa from maize (Zea mays) coleoptiles and gave good results. These techniques can be used for isolation of any water-soluble protein from plants.

KW - Ammonium sulfate precipitation

KW - Hydrophobic interaction chromatography

KW - Phenol separation

KW - Polyethylene glycol fractionation

KW - Protein isolation

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JF - Plant Physiology and Biochemistry

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ER -

Separation of proteins from phenols in cereal leaf extract by hydrophobic interaction- ammonium sulfate fractionation

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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