Sequence of canine COL1A2 cDNA: Nucleotide substitutions affecting the cyanogen bromide peptide map of the α2(I) chain

Bonnie G. Campbell, Joyce A.M. Wootton, James N. MacLeod, Ronald R. Minor

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


The α2 chain of canine type I collagen was characterized with both sequence analysis of COL1A2 cDNA and chemical analysis of α2(I) chains. The complete sequence of canine COL1A2 cDNA was determined from reverse- transcribed and polymerase chain reaction-amplified total RNA from cultured skin fibroblasts. Pepsin-digested and cyanogen bromide-digested type I collagen peptides were analyzed with chromatography, gel electrophoresis, and mass spectrometry. Identity between the sequences of canine and human COL1A2 cDNA was 90.9%, predicting conservation of the 3 cysteine residues required for C-propeptide registration and of cleavage sites for signal peptidase, procollagen N-proteinase, vertebrate collagenase, and procollagen C- proteinase. Conservation of all 50 lysine residues was also predicted, including preservation of the 1:2 asymmetry in the X:Y distribution of the 31 lysine residues in the α2(I) triple helix. The human and canine sequences differed in the location of Y-position proline residues and the presence of two unique canine cyanogen bromide peptides, α2 CB3b and α2 CB3c,5. Knowledge of the conserved and unique features of canine COL1A2 will be valuable for analysis of the expression, synthesis, and structure of type I collagen as well as studies of canine osteogenesis imperfecta.

Original languageEnglish
Pages (from-to)67-75
Number of pages9
JournalArchives of Biochemistry and Biophysics
Issue number1
StatePublished - Sep 1 1998

Bibliographical note

Funding Information:
This work was supported by Research Grants AR08428 (B.G.C.) and AR20793 (R.R.M.) from the National Institutes of Health and by a grant from The IAMS Company, Lewisburg, Ohio. The authors thank Drs. Darwin Prockop, Helena Kuivaniemi, and Gerard Tromp for supplying the human COL1A2 cDNA and Dr. Da Nian Gu, Ms. Barbara Hover, and Mr. Jonathon Tetreault for their contributions to this study.


  • COL1A2
  • Canine
  • Cyanogen bromide
  • Type I collagen
  • α2(I)

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology


Dive into the research topics of 'Sequence of canine COL1A2 cDNA: Nucleotide substitutions affecting the cyanogen bromide peptide map of the α2(I) chain'. Together they form a unique fingerprint.

Cite this