Serum amyloid A (SAA): Influence on HDL-mediated cellular cholesterol efflux

C. L. Banka, T. Yuan, M. C. De Beer, M. Kindy, L. K. Curtiss, F. C. De Beer

Research output: Contribution to journalArticlepeer-review

182 Scopus citations

Abstract

Normal high density lipoprotein (N-HDL) is remodeled during acute phase (AP) reactions by the association of serum amyloid A (SAA) and the depletion of apolipoprotein (apo) A-I. To determine the impact of this remodeling on HDL function, the capacities of N-HDL and AP-HDL to associate with and promote cholesterol efflux from human monocytic THP-1 cells were compared. THP-1 cells preferentially bound AP-HDL compared with N-HDL. Examination of the AP-HDL particles bound to THP-1 cells revealed a disproportionate association of an apoSAA-enriched, apoA-I-depleted subpopulation compared with the composition of the starting material. However, N-HDL and AP-HDL promoted cholesterol efflux from THP-1 cells equally efficiently and in a dose-dependent manner. When N-HDL was experimentally remodeled with apoSAA to achieve an apoprotein composition similar to that of the preferentially bound particles, cellular cholesterol efflux was reduced by 30%. The remodelling of HDL with apoSAA during the acute phase reaction alters cholesterol efflux only when apoSAA constitutes more than 50% of the HDL protein.

Original languageEnglish
Pages (from-to)1058-1065
Number of pages8
JournalJournal of Lipid Research
Volume36
Issue number5
StatePublished - 1995

Keywords

  • acute phase proteins
  • apolipoproteins
  • reverse cholesterol transport

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

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