Shoc2-tranduced ERK1/2 motility signals - Novel insights from functional genomics

Myoungkun Jeoung, Eun Ryoung Jang, Jinpeng Liu, Chi Wang, Eric C. Rouchka, Xiaohong Li, Emilia Galperin

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


The extracellular signal-regulated kinase 1 and 2 (ERK1/2) pathway plays a central role in defining various cellular fates. Scaffold proteins modulating ERK1/2 activity control growth factor signals transduced by the pathway. Here, we analyzed signals transduced by Shoc2, a critical positive modulator of ERK1/2 activity. We found that loss of Shoc2 results in impaired cell motility and delays cell attachment. As ERKs control cellular fates by stimulating transcriptional response, we hypothesized that the mechanisms underlying changes in cell adhesion could be revealed by assessing the changes in transcription of Shoc2-depleted cells. Using quantitative RNA-seq analysis, we identified 853 differentially expressed transcripts. Characterization of the differentially expressed genes showed that Shoc2 regulates the pathway at several levels, including expression of genes controlling cell motility, adhesion, crosstalk with the transforming growth factor beta (TGFβ) pathway, and expression of transcription factors. To understand the mechanisms underlying delayed attachment of cells depleted of Shoc2, changes in expression of the protein of extracellular matrix (lectin galactoside-binding soluble 3-binding protein; LGALS3BP) were functionally analyzed. We demonstrated that delayed adhesion of the Shoc2-depleted cells is a result of attenuated expression and secretion of LGALS3BP. Together our results suggest that Shoc2 regulates cell motility by modulating ERK1/2 signals to cell adhesion.

Original languageEnglish
Pages (from-to)448-459
Number of pages12
JournalCellular Signalling
Issue number5
StatePublished - May 1 2016

Bibliographical note

Publisher Copyright:
© 2016 Elsevier Inc.


  • Adhesion
  • ERK1/2
  • Motility
  • Shoc2 scaffold
  • Transcription

ASJC Scopus subject areas

  • Cell Biology


Dive into the research topics of 'Shoc2-tranduced ERK1/2 motility signals - Novel insights from functional genomics'. Together they form a unique fingerprint.

Cite this