Side chain packing below the fusion peptide strongly modulates triggering of the Hendra virus F protein

Everett Clinton Smith, Rebecca Ellis Dutch

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Triggering of the Hendra virus fusion (F) protein is required to initiate the conformational changes which drive membrane fusion, but the factors which control triggering remain poorly understood. Mutation of a histidine predicted to lie near the fusion peptide to alanine greatly reduced fusion despite wild-type cell surface expression levels, while asparagine substitution resulted in a moderate restoration in fusion levels. Slowed kinetics of six-helix bundle formation, as judged by sensitivity to heptad repeat B-derived peptides, was observed for all H372 mutants. These data suggest that side chain packing beneath the fusion peptide is an important regulator of Hendra virus F triggering.

Original languageEnglish
Pages (from-to)10928-10932
Number of pages5
JournalJournal of Virology
Volume84
Issue number20
DOIs
StatePublished - Oct 2010

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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