TY - JOUR
T1 - Side chain packing below the fusion peptide strongly modulates triggering of the Hendra virus F protein
AU - Smith, Everett Clinton
AU - Dutch, Rebecca Ellis
PY - 2010/10
Y1 - 2010/10
N2 - Triggering of the Hendra virus fusion (F) protein is required to initiate the conformational changes which drive membrane fusion, but the factors which control triggering remain poorly understood. Mutation of a histidine predicted to lie near the fusion peptide to alanine greatly reduced fusion despite wild-type cell surface expression levels, while asparagine substitution resulted in a moderate restoration in fusion levels. Slowed kinetics of six-helix bundle formation, as judged by sensitivity to heptad repeat B-derived peptides, was observed for all H372 mutants. These data suggest that side chain packing beneath the fusion peptide is an important regulator of Hendra virus F triggering.
AB - Triggering of the Hendra virus fusion (F) protein is required to initiate the conformational changes which drive membrane fusion, but the factors which control triggering remain poorly understood. Mutation of a histidine predicted to lie near the fusion peptide to alanine greatly reduced fusion despite wild-type cell surface expression levels, while asparagine substitution resulted in a moderate restoration in fusion levels. Slowed kinetics of six-helix bundle formation, as judged by sensitivity to heptad repeat B-derived peptides, was observed for all H372 mutants. These data suggest that side chain packing beneath the fusion peptide is an important regulator of Hendra virus F triggering.
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U2 - 10.1128/JVI.01108-10
DO - 10.1128/JVI.01108-10
M3 - Article
C2 - 20702638
AN - SCOPUS:77957196709
SN - 0022-538X
VL - 84
SP - 10928
EP - 10932
JO - Journal of Virology
JF - Journal of Virology
IS - 20
ER -