TY - JOUR
T1 - Simultaneous glutamate recordings in the frontal cortex network with multisite biomorphic microelectrodes
T2 - New tools for ADHD research
AU - Miller, Erin M.
AU - Quintero, Jorge E.
AU - Pomerleau, François
AU - Huettl, Peter
AU - Gerhardt, Greg A.
AU - Glaser, Paul E.A.
N1 - Publisher Copyright:
© 2015 .
PY - 2015/10/30
Y1 - 2015/10/30
N2 - Background: The aberrant regulation of glutamate has been implicated in numerous psychiatric disorders including drug addiction and attention-deficit/hyperactivity disorder. To understand glutamate signaling and its role in facilitating disease, tools to directly measure glutamate in a complex, neural network are needed. New method: The development of a ceramic-based, dual-sided, biomorphic microelectrode array with four recording sites on each side to facilitate a more detailed measurement of glutamate in awake, behaving rodents. Results: In vitro calibrations of these biosensors showed selective and specific responses to glutamate. In awake rats, these biomorphic electrode arrays enabled the concurrent evaluation of glutamate in a network, the frontal cortex: including the cingulate, prelimbic, infralimbic and dorsal peduncle regions. Regions within the frontal cortex exhibited varying phasic glutamate patterns in awake animals.Comparison with existing method: Existing methodologies to measure glutamate neurotransmission employ single-sided biosensors or biosensors capable of measuring neurochemicals at only one location in space. Conclusions: Multi-site, biomorphic neurochemical biosensors provide a method for simultaneously measuring glutamate in multiple areas of a neural network in the brain.
AB - Background: The aberrant regulation of glutamate has been implicated in numerous psychiatric disorders including drug addiction and attention-deficit/hyperactivity disorder. To understand glutamate signaling and its role in facilitating disease, tools to directly measure glutamate in a complex, neural network are needed. New method: The development of a ceramic-based, dual-sided, biomorphic microelectrode array with four recording sites on each side to facilitate a more detailed measurement of glutamate in awake, behaving rodents. Results: In vitro calibrations of these biosensors showed selective and specific responses to glutamate. In awake rats, these biomorphic electrode arrays enabled the concurrent evaluation of glutamate in a network, the frontal cortex: including the cingulate, prelimbic, infralimbic and dorsal peduncle regions. Regions within the frontal cortex exhibited varying phasic glutamate patterns in awake animals.Comparison with existing method: Existing methodologies to measure glutamate neurotransmission employ single-sided biosensors or biosensors capable of measuring neurochemicals at only one location in space. Conclusions: Multi-site, biomorphic neurochemical biosensors provide a method for simultaneously measuring glutamate in multiple areas of a neural network in the brain.
KW - Biosensors
KW - Frontal cortex
KW - Glutamate
KW - Microelectrode array
KW - Neural network
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U2 - 10.1016/j.jneumeth.2015.01.018
DO - 10.1016/j.jneumeth.2015.01.018
M3 - Article
C2 - 25614383
AN - SCOPUS:84952628124
SN - 0165-0270
VL - 252
SP - 75
EP - 79
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
ER -