Simultaneous measurements of ascorbate and glutamate in vivo in the rat brain using carbon fiber nanocomposite sensors and microbiosensor arrays

Nuno R. Ferreira; Ana Ledo; João Laranjinha; Greg A. Gerhardt; Rui M. Barbosa

doi:10.1016/j.bioelechem.2018.01.009

Simultaneous measurements of ascorbate and glutamate in vivo in the rat brain using carbon fiber nanocomposite sensors and microbiosensor arrays

Nuno R. Ferreira, Ana Ledo, João Laranjinha, Greg A. Gerhardt, Rui M. Barbosa

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Nanocomposite sensors consisting of carbon fiber microelectrodes modified with Nafion® and carbon nanotubes, and ceramic-based microelectrode biosensor arrays were used to measure ascorbate and glutamate in the brain with high spatial, temporal and chemical resolution. Nanocomposite sensors displayed electrocatalytic properties towards ascorbate oxidation, translated into a negative shift from +0.20 V to −0.05 V vs. Ag/AgCl, as well as a significant increase (10-fold) of electroactive surface area. The estimated average basal concentration of ascorbate in vivo in the CA1, CA3 and dentate gyrus (DG) sub regions of the hippocampus were 276 ± 60 μM (n = 10), 183 ± 30 μM (n = 10) and 133 ± 42 μM (n = 10), respectively. The glutamate microbiosensor arrays showed a high sensitivity of 5.3 ± 0.8 pA μM⁻¹ (n = 18), and LOD of 204 ± 32 nM (n = 10), and t_50% response time of 0.9 ± 0.02 s (n = 6) and high selectivity against major interferents. The simultaneous and real-time measurements of glutamate and ascorbate in the hippocampus of anesthetized rats following local stimulus with KCl or glutamate revealed a dynamic interaction between the two neurochemicals.

Original language	English
Pages (from-to)	142-150
Number of pages	9
Journal	Bioelectrochemistry
Volume	121
DOIs	https://doi.org/10.1016/j.bioelechem.2018.01.009
State	Published - Jun 2018

Bibliographical note

Funding Information:
This work was funded by the European Regional Development Fund (ERDF), through the Centro 2020 Regional Operational Program: project CENTRO-01-0145-FEDER-000012-HealthyAging2020 , the COMPETE 2020 - Operational Program for Competitiveness and Internationalization, and the Portuguese national funds via FCT – Fundação para a Ciência e a Tecnologia , I.P.: project POCI-01-0145-FEDER-007440 .

Keywords

Ascorbate
Carbon nanotubes
Glutamate
In vivo monitoring
Microelectrode arrays

ASJC Scopus subject areas

Biophysics
Physical and Theoretical Chemistry
Electrochemistry

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10.1016/j.bioelechem.2018.01.009

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title = "Simultaneous measurements of ascorbate and glutamate in vivo in the rat brain using carbon fiber nanocomposite sensors and microbiosensor arrays",

abstract = "Nanocomposite sensors consisting of carbon fiber microelectrodes modified with Nafion{\textregistered} and carbon nanotubes, and ceramic-based microelectrode biosensor arrays were used to measure ascorbate and glutamate in the brain with high spatial, temporal and chemical resolution. Nanocomposite sensors displayed electrocatalytic properties towards ascorbate oxidation, translated into a negative shift from +0.20 V to −0.05 V vs. Ag/AgCl, as well as a significant increase (10-fold) of electroactive surface area. The estimated average basal concentration of ascorbate in vivo in the CA1, CA3 and dentate gyrus (DG) sub regions of the hippocampus were 276 ± 60 μM (n = 10), 183 ± 30 μM (n = 10) and 133 ± 42 μM (n = 10), respectively. The glutamate microbiosensor arrays showed a high sensitivity of 5.3 ± 0.8 pA μM−1 (n = 18), and LOD of 204 ± 32 nM (n = 10), and t50% response time of 0.9 ± 0.02 s (n = 6) and high selectivity against major interferents. The simultaneous and real-time measurements of glutamate and ascorbate in the hippocampus of anesthetized rats following local stimulus with KCl or glutamate revealed a dynamic interaction between the two neurochemicals.",

keywords = "Ascorbate, Carbon nanotubes, Glutamate, In vivo monitoring, Microelectrode arrays",

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doi = "10.1016/j.bioelechem.2018.01.009",

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AU - Ferreira, Nuno R.

AU - Ledo, Ana

AU - Laranjinha, João

AU - Gerhardt, Greg A.

AU - Barbosa, Rui M.

PY - 2018/6

Y1 - 2018/6

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Simultaneous measurements of ascorbate and glutamate in vivo in the rat brain using carbon fiber nanocomposite sensors and microbiosensor arrays

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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