The human health benefits attributed to turmeric/curcumin spice has resulted in its wide utilization as a dietary supplement for companion pets and other animals including horses. While the quantification of free curcuminoids (curcumin, demethoxycurcumin, bisdemethoxycurcumin) and their phase-2 metabolites (curcumin-O-sulfate, curcumin-O-glucuronide) have been extensively investigated in human and rodent biological samples (primarily plasma and serum), there is lack of similar data for horses. Herein, we report a validated LC-ESI–MS/MS method for the simultaneous quantification of the aforementioned free curcuminoids and their metabolites in equine plasma. The linearity of the aforementioned curcuminoids and curcumin-O-sulfate was in the range of 0.5–1000 ng/mL and 1–1000 ng/mL for curcumin-O-glucuronide with 85–115% accuracy and <15% precision in equine plasma. The method was validated based on US FDA criteria and applied to characterize the pharmacokinetics of curcumin-O-sulfate in equine plasma.
|Number of pages
|Journal of Pharmaceutical and Biomedical Analysis
|Published - May 30 2018
Bibliographical noteFunding Information:
This project was conducted using a mass spectrometry instrument located in the Rhode Island NSF EPSCoR research facility, supported in part by the National Science Foundation EPSCoR Cooperative Agreement #EPS-1004057.
© 2018 Elsevier B.V.
ASJC Scopus subject areas
- Analytical Chemistry
- Pharmaceutical Science
- Drug Discovery
- Clinical Biochemistry