Simultaneous quantification of free curcuminoids and their metabolites in equine plasma by LC-ESI–MS/MS

Yongqiang Liu; Melissa Siard; Amanda Adams; Michael L. Keowen; Travis K. Miller; Frank Garza; Frank M. Andrews; Navindra P. Seeram

doi:10.1016/j.jpba.2018.03.014

Simultaneous quantification of free curcuminoids and their metabolites in equine plasma by LC-ESI–MS/MS

Yongqiang Liu, Melissa Siard, Amanda Adams, Michael L. Keowen, Travis K. Miller, Frank Garza, Frank M. Andrews, Navindra P. Seeram

Veterinary Science

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

The human health benefits attributed to turmeric/curcumin spice has resulted in its wide utilization as a dietary supplement for companion pets and other animals including horses. While the quantification of free curcuminoids (curcumin, demethoxycurcumin, bisdemethoxycurcumin) and their phase-2 metabolites (curcumin-O-sulfate, curcumin-O-glucuronide) have been extensively investigated in human and rodent biological samples (primarily plasma and serum), there is lack of similar data for horses. Herein, we report a validated LC-ESI–MS/MS method for the simultaneous quantification of the aforementioned free curcuminoids and their metabolites in equine plasma. The linearity of the aforementioned curcuminoids and curcumin-O-sulfate was in the range of 0.5–1000 ng/mL and 1–1000 ng/mL for curcumin-O-glucuronide with 85–115% accuracy and <15% precision in equine plasma. The method was validated based on US FDA criteria and applied to characterize the pharmacokinetics of curcumin-O-sulfate in equine plasma.

Original language	English
Pages (from-to)	31-39
Number of pages	9
Journal	Journal of Pharmaceutical and Biomedical Analysis
Volume	154
DOIs	https://doi.org/10.1016/j.jpba.2018.03.014
State	Published - May 30 2018

Bibliographical note

Publisher Copyright:
© 2018 Elsevier B.V.

Funding

This project was conducted using a mass spectrometry instrument located in the Rhode Island NSF EPSCoR research facility, supported in part by the National Science Foundation EPSCoR Cooperative Agreement #EPS-1004057.

Funders	Funder number
National Science Foundation/EPSCoR	-1004057
Rhode Island NSF EPSCoR

Keywords

Curcuminoids
Equine
LC–MS/MS
Metabolites
Pharmacokinetics
Plasma

ASJC Scopus subject areas

Analytical Chemistry
Pharmaceutical Science
Drug Discovery
Spectroscopy
Clinical Biochemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jpba.2018.03.014

Cite this

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title = "Simultaneous quantification of free curcuminoids and their metabolites in equine plasma by LC-ESI–MS/MS",

abstract = "The human health benefits attributed to turmeric/curcumin spice has resulted in its wide utilization as a dietary supplement for companion pets and other animals including horses. While the quantification of free curcuminoids (curcumin, demethoxycurcumin, bisdemethoxycurcumin) and their phase-2 metabolites (curcumin-O-sulfate, curcumin-O-glucuronide) have been extensively investigated in human and rodent biological samples (primarily plasma and serum), there is lack of similar data for horses. Herein, we report a validated LC-ESI–MS/MS method for the simultaneous quantification of the aforementioned free curcuminoids and their metabolites in equine plasma. The linearity of the aforementioned curcuminoids and curcumin-O-sulfate was in the range of 0.5–1000 ng/mL and 1–1000 ng/mL for curcumin-O-glucuronide with 85–115% accuracy and <15% precision in equine plasma. The method was validated based on US FDA criteria and applied to characterize the pharmacokinetics of curcumin-O-sulfate in equine plasma.",

keywords = "Curcuminoids, Equine, LC–MS/MS, Metabolites, Pharmacokinetics, Plasma",

author = "Yongqiang Liu and Melissa Siard and Amanda Adams and Keowen, {Michael L.} and Miller, {Travis K.} and Frank Garza and Andrews, {Frank M.} and Seeram, {Navindra P.}",

note = "Publisher Copyright: {\textcopyright} 2018 Elsevier B.V.",

year = "2018",

month = may,

day = "30",

doi = "10.1016/j.jpba.2018.03.014",

language = "English",

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T1 - Simultaneous quantification of free curcuminoids and their metabolites in equine plasma by LC-ESI–MS/MS

AU - Liu, Yongqiang

AU - Siard, Melissa

AU - Adams, Amanda

AU - Keowen, Michael L.

AU - Miller, Travis K.

AU - Garza, Frank

AU - Andrews, Frank M.

AU - Seeram, Navindra P.

PY - 2018/5/30

Y1 - 2018/5/30

N2 - The human health benefits attributed to turmeric/curcumin spice has resulted in its wide utilization as a dietary supplement for companion pets and other animals including horses. While the quantification of free curcuminoids (curcumin, demethoxycurcumin, bisdemethoxycurcumin) and their phase-2 metabolites (curcumin-O-sulfate, curcumin-O-glucuronide) have been extensively investigated in human and rodent biological samples (primarily plasma and serum), there is lack of similar data for horses. Herein, we report a validated LC-ESI–MS/MS method for the simultaneous quantification of the aforementioned free curcuminoids and their metabolites in equine plasma. The linearity of the aforementioned curcuminoids and curcumin-O-sulfate was in the range of 0.5–1000 ng/mL and 1–1000 ng/mL for curcumin-O-glucuronide with 85–115% accuracy and <15% precision in equine plasma. The method was validated based on US FDA criteria and applied to characterize the pharmacokinetics of curcumin-O-sulfate in equine plasma.

AB - The human health benefits attributed to turmeric/curcumin spice has resulted in its wide utilization as a dietary supplement for companion pets and other animals including horses. While the quantification of free curcuminoids (curcumin, demethoxycurcumin, bisdemethoxycurcumin) and their phase-2 metabolites (curcumin-O-sulfate, curcumin-O-glucuronide) have been extensively investigated in human and rodent biological samples (primarily plasma and serum), there is lack of similar data for horses. Herein, we report a validated LC-ESI–MS/MS method for the simultaneous quantification of the aforementioned free curcuminoids and their metabolites in equine plasma. The linearity of the aforementioned curcuminoids and curcumin-O-sulfate was in the range of 0.5–1000 ng/mL and 1–1000 ng/mL for curcumin-O-glucuronide with 85–115% accuracy and <15% precision in equine plasma. The method was validated based on US FDA criteria and applied to characterize the pharmacokinetics of curcumin-O-sulfate in equine plasma.

KW - Curcuminoids

KW - Equine

KW - LC–MS/MS

KW - Metabolites

KW - Pharmacokinetics

KW - Plasma

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Simultaneous quantification of free curcuminoids and their metabolites in equine plasma by LC-ESI–MS/MS

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

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