Simultaneous quantification of free curcuminoids and their metabolites in equine plasma by LC-ESI–MS/MS

Yongqiang Liu, Melissa Siard, Amanda Adams, Michael L. Keowen, Travis K. Miller, Frank Garza, Frank M. Andrews, Navindra P. Seeram

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

The human health benefits attributed to turmeric/curcumin spice has resulted in its wide utilization as a dietary supplement for companion pets and other animals including horses. While the quantification of free curcuminoids (curcumin, demethoxycurcumin, bisdemethoxycurcumin) and their phase-2 metabolites (curcumin-O-sulfate, curcumin-O-glucuronide) have been extensively investigated in human and rodent biological samples (primarily plasma and serum), there is lack of similar data for horses. Herein, we report a validated LC-ESI–MS/MS method for the simultaneous quantification of the aforementioned free curcuminoids and their metabolites in equine plasma. The linearity of the aforementioned curcuminoids and curcumin-O-sulfate was in the range of 0.5–1000 ng/mL and 1–1000 ng/mL for curcumin-O-glucuronide with 85–115% accuracy and <15% precision in equine plasma. The method was validated based on US FDA criteria and applied to characterize the pharmacokinetics of curcumin-O-sulfate in equine plasma.

Original languageEnglish
Pages (from-to)31-39
Number of pages9
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume154
DOIs
StatePublished - May 30 2018

Bibliographical note

Funding Information:
This project was conducted using a mass spectrometry instrument located in the Rhode Island NSF EPSCoR research facility, supported in part by the National Science Foundation EPSCoR Cooperative Agreement #EPS-1004057.

Publisher Copyright:
© 2018 Elsevier B.V.

Keywords

  • Curcuminoids
  • Equine
  • LC–MS/MS
  • Metabolites
  • Pharmacokinetics
  • Plasma

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

Fingerprint

Dive into the research topics of 'Simultaneous quantification of free curcuminoids and their metabolites in equine plasma by LC-ESI–MS/MS'. Together they form a unique fingerprint.

Cite this