Single-chain antibodies against a plant viral RNA-dependent RNA polymerase confer virus resistance

Ka John Boonrod; Danuta Galetzka; Peter D. Nagy; Udo Conrad; Gabi Krczal

doi:10.1038/nbt983

Single-chain antibodies against a plant viral RNA-dependent RNA polymerase confer virus resistance

Ka John Boonrod, Danuta Galetzka, Peter D. Nagy, Udo Conrad, Gabi Krczal

Plant Pathology

Research output: Contribution to journal › Article › peer-review

88 Scopus citations

Abstract

Crop loss due to viral diseases is still a major problem for agriculture today. We present a strategy to achieve virus resistance based on the expression of single-chain Fv fragments (scFvs) against a conserved domain in a plant viral RNA-dependent RNA polymerase (RdRp), a key enzyme in virus replication. The selected scFvs inhibited complementary RNA synthesis of different plant virus RdRps in vitro and virus replication in planta. Moreover, the scFvs also bound to the RdRp of the distantly related hepatitis C virus. T₁ and T₂ progeny of transgenic lines of Nicotiana benthamiana expressing different scFvs either in the cytosol or in the endoplasmic reticulum showed varying degrees of resistance against four plant viruses from different genera, three of which belong to the Tombusviridae family. Virus resistance based on antibodies to RdRps adds another tool to the repertoire for combating plant viruses.

Original language	English
Pages (from-to)	856-862
Number of pages	7
Journal	Nature Biotechnology
Volume	22
Issue number	7
DOIs	https://doi.org/10.1038/nbt983
State	Published - Jul 2004

Bibliographical note

Funding Information:
We thank Judit Pogany for providing CNV RdRp and K.S. Rajendran for providing TCV RdRp. We also thank Gudrun Mönke for anti-FUS3-scFv and Ralf Bartenschlager for the HCV NS5B preparation. We thank Jan-Peter Nap (Hanze Hogeschool, Groningen), Michael Wassenegger and Pascal Cobanov for critically reviewing this manuscript and for helpful discussion. We are grateful to Sasithorn Chotiwutmontri and Claudia Eisenhauer for technical assistance. This work was supported by a grant from the Stiftung Rheinland-Pfalz für Innovation awarded to G.K. and in part by a National Science Foundation award to P.D.N.

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Molecular Medicine
Biomedical Engineering

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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abstract = "Crop loss due to viral diseases is still a major problem for agriculture today. We present a strategy to achieve virus resistance based on the expression of single-chain Fv fragments (scFvs) against a conserved domain in a plant viral RNA-dependent RNA polymerase (RdRp), a key enzyme in virus replication. The selected scFvs inhibited complementary RNA synthesis of different plant virus RdRps in vitro and virus replication in planta. Moreover, the scFvs also bound to the RdRp of the distantly related hepatitis C virus. T1 and T2 progeny of transgenic lines of Nicotiana benthamiana expressing different scFvs either in the cytosol or in the endoplasmic reticulum showed varying degrees of resistance against four plant viruses from different genera, three of which belong to the Tombusviridae family. Virus resistance based on antibodies to RdRps adds another tool to the repertoire for combating plant viruses.",

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AU - Conrad, Udo

AU - Krczal, Gabi

N1 - Funding Information: We thank Judit Pogany for providing CNV RdRp and K.S. Rajendran for providing TCV RdRp. We also thank Gudrun Mönke for anti-FUS3-scFv and Ralf Bartenschlager for the HCV NS5B preparation. We thank Jan-Peter Nap (Hanze Hogeschool, Groningen), Michael Wassenegger and Pascal Cobanov for critically reviewing this manuscript and for helpful discussion. We are grateful to Sasithorn Chotiwutmontri and Claudia Eisenhauer for technical assistance. This work was supported by a grant from the Stiftung Rheinland-Pfalz für Innovation awarded to G.K. and in part by a National Science Foundation award to P.D.N.

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Single-chain antibodies against a plant viral RNA-dependent RNA polymerase confer virus resistance

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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